Open Access
Research (Published online: 19-02-2018)
20. Comparative ovicidal activity of Moringa oleifera leaf extracts on Fasciola gigantica eggs
Ahmed G. Hegazi, Kadria N. Abdel Megeed, Soad E. Hassan, M. M. Abdelaziz, Nagwa I. Toaleb, Eman E. El Shanawany and Dina Aboelsoued
Veterinary World, 11(2): 215-220

Ahmed G. Hegazi: Department of Zoonotic Diseases, National Research Centre, Dokki, Giza, Egypt.
Kadria N. Abdel Megeed: Department of Parasitology and Animal Diseases, National Research Centre, Dokki, Giza, Egypt.
Soad E. Hassan: Department of Parasitology and Animal Diseases, National Research Centre, Dokki, Giza, Egypt.
M. M. Abdelaziz: Department of Parasitology and Animal Diseases, National Research Centre, Dokki, Giza, Egypt.
Nagwa I. Toaleb: Department of Parasitology and Animal Diseases, National Research Centre, Dokki, Giza, Egypt.
Eman E. El Shanawany: Department of Parasitology and Animal Diseases, National Research Centre, Dokki, Giza, Egypt.
Dina Aboelsoued: Department of Parasitology and Animal Diseases, National Research Centre, Dokki, Giza, Egypt.

doi: 10.14202/vetworld.2018.215-220

Share this article on [Facebook] [LinkedIn]

Article history: Received: 14-09-2017, Accepted: 10-01-2018, Published online: 19-02-2018

Corresponding author: Dina Aboelsoued

E-mail: Dr.Dina.Aboelsoued@gmail.com

Citation: Hegazi AG, Abdel Megeed KN, Hassan SE, Abdelaziz MM, Toaleb NI, El Shanawany EE, Aboelsoued D (2018) Comparative ovicidal activity of Moringa oleifera leaf extracts on Fasciola gigantica eggs, Veterinary World, 11(2): 215-220.
Abstract

Background: Fasciolosis is an important zoonotic disease affecting the productive performance of farm animals in Egypt.

Aim: The aim of the present study was comparing the ovicidal effect of different extracts as an alcoholic (Methanolic and Ethanolic) and aqueous Moringa oleifera leaf extracts on Fasciola gigantica non-embryonated and developed eggs.

Materials and Methods: Tested concentrations of extracts ranged from 12.5 to 800 mg/ml. Nitroxynil was used as reference drug with a dose of 100 mg/ml.

Results: M. oleifera alcoholic and aqueous extracts showed a concentration-dependent ovicidal effect on F. gigantica non-embryonated and developed eggs. Based on LC50 values, water extract showed the highest ovicidal activity since it registered the lowest values of 2.6 mg/ml on non-embryonated eggs. Non-embryonated eggs were more susceptible to aqueous extract than developed eggs. On the other hand, the developed eggs were more susceptible to ethanolic extract than non-embryonated eggs even the lowest LC50 (12.38 mg/ml).

Conclusion: M. oleifera leaf extracts especially aqueous extract could be a promising step in the field of controlling fascioliasis. Further, in vivo studies are needed to enlighten the therapeutic potential of M. oleifera extracts in treating F. gigantica infection.

Keywords: Fasciola gigantica, leaf extract, Moringa oleifera, nitroxynil, ovicidal activity.

References

1. Shokier, K.M., Aboelhadid, S.M. and Waleed, M.A. (2013) Efficacy of five anthelmintics against a natural Fasciola species infection in cattle. BJBAS, 2: 4-5. [Crossref]

2. Schweizer, G., Braun, U., Deplazes, P. and Torgerson, P.R. (2005) Estimating the financial losses due to bovine fasciolosis in Switzerland. Vet. Rec., 157: 188-193. [Crossref] [PubMed]

3. Charlier, J., Duchateau, L., Claerebout, E., Williams, D. and Vercruysse, J. (2007) Associations between anti-Fasciola hepatica antibody levels in bulk-tank milk samples and production parameters in dairy herds. Prev. Vet. Med., 78: 57-66. [Crossref] [PubMed]

4. Veerakumari, L. (2015) Botanical anthelmintics. Asian J. Sci. Tech., 6: 1881-1894.

5. Bauri, R.K., Tigga, M.N. and Kullu S.S. (2015) A review on use of medicinal plants to control parasites. Ind. J. Natl. Prod. Resour., 6: 268-277.

6. Devendra, B.N., Srinivas, N., Talluri, P.S. and Latha, P.S. (2011) Antimicrobial Activity of Moringa oleifera Lam., leaf extract, against selected bacterial and fungal strains. Int. J. Pharm. BioSci., 2: 13-18.

7. Nilani, P., Pinaka, M.K., Duraisamy, B., Dhamodaran, P. and Jeyaprakash, M.R. (2012) Anthelmintic activity of Moringa Oleifera seed oil - Validation of traditional use. J. Adv. Sci. Res., 3: 65-66.

8. Rastogi, T., Bhutda, V., Moon, K., Aswar, P.B. and Khadabadi, S.S. (2009) Comparative studies on anthelmintic activity of Moringa oleifera and Vitex negundo. Asian J. Res. Chem., 2: 181-182.

9. Konmy, B.B.S., Olounlade, P.A., Doko-allou, S., Azando, E.B.V. and Hounzangbe-Adote, S.E.G. (2016) A review on phytochemistry and pharmacology of Moringa oleifera leaves (Moringaceae). J. Pharm. Phytochem., 5: 325-330.

10. Martinez-Gonzalez, C.L., Martinez, L., Martinez-Ortiz, E.J., Gonzalez-Trujano, M.E., Deciga-Campos, M., Ventura-Martinez, R. and Diaz-Reval, I. (2017) Moringa oleifera, a species with potential analgesic and anti-inflammatory activities. Biomed. Pharmacother., 87: 482-488. [Crossref] [PubMed]

11. Mbikay, M. (2012), Therapeutic potential of Moringa oleifera leaves in chronic hyper-glycemicemia and dyslipidemia: A review. Front. Pharmacol., 3: 1-37. [Crossref] [PubMed] [PMC]

12. Fairweather, I., McShane, D., Shaw, L., Ellison, S.E., O'Hagan, N.T., York, E.A., Trudgett, A. and Brennan, G.P. (2012), Development of an egg hatch assay for the diagnosis of triclabendazole resistance in Fasciola hepatica: Proof of concept. Vet. Parasitol., 183: 249-259. [Crossref]

13. Arafa, W.A., Shokeir, K.M. and Khateib, A.M. (2015) Comparing an in vivo egg reduction test and in vitro egg hatching assay for different anthelmintics against Fasciola species, in cattle. Vet. Parasitol., 214: 152-158. [Crossref]

14. Canevari, J., Ceballos, L., Sanabria, R. and Romero, J. (2014) Testing albendazole resistance in Fasciola hepatica: Validation of an egg hatch test with isolates from South America and the United Kingdom. J. Helminthol., 88: 286-292. [Crossref]

15. SPSS 15.0. (2006) Statistical Package for Social Science, SPSS for Windows Evaluation Version. Standard Version, Copyright SPSS Inc., 1989-2006. Buckingham.

16. Pessoa, L.M., Movais, S.M., Bevilaqaliua, C.M.L. and Luciano, J.H.S. (2002) Anthelmintic activity of essential oil of Ocimum gratissimum Linn. and eugenol against Haemonchus contortus. Vet. Parasitol., 109: 59-63. [Crossref]

17. Pone, W.J., Mpoame, M., Bilong, B.C.F. and Kerboeuf, D. (2005) Etude compare in vitro de l'activite nematodicide de l'extrait ethanolique de la poudre d'ecorce de Canthium mannii (Rubiaceae) et du Mebendazole. Rev. Med. Vet., 156: 633-636.

18. Pone, W.J., Bilong, B.C.F., Mpoame, M., Ngwa, C.F. and Coles, C.G. (2006) In vitro activity of ethanolic, cold and hot water extracts of the stem bark of Canthium mannii (Rubiaceae) stem on Ancylostoma caninum eggs. East Cent. Afr. J. Pharm. Sci., 9: 14-18.

19. Peter, W. and Deogracious, O. (2006) The in-vitro ascaricidal activity of selected indigenous medicinal plants used in ethno veterinary practices in Uganda. Afr. J. Trad. Cam., 3: 94-103.

20. Fatima, T., Sajid, M.S., Hassan, M.J.U., Siddique, R.M. and Iqbal, Z. (2014) Phytomedicinal value of Moringa oleifera with special reference to antiparasitics. Pak. J. Agric. Sci., 51: 251-262.

21. Ali, S.A., Mohamed, A.H., Gameel, A.A. and Hassan, T. (2010) Protective Effect of Moringa Oleifera Leaves Aqueous Extract against Carbon Tetrachloride Induced Hepatotoxicity in Rats. 14th ed. Science Congress Faculty of Veterinary Medicine, Assiut, Egypt. p327-336.

22. Cabardo, D.E. and Portugaliza, H.P. (2017) Anthelmintic activity of Moringa oleifera seed aqueous and ethanolic extracts against Haemonchus contortus eggs and third stage larvae. Int. J. Vet. Sci. Med.,5: 30-34. [Crossref]

23. Vargas-Maga-a, J.J., Torres-Acosta, J.F.J., Aguilar-Caballero, A.J., Sandoval-Castro, C.A., Hoste, H. and Chan-Perez, J.I. (2014) Anthelmintic activity of acetone-water extracts against Haemonchus contortus eggs: Interactions between tannins and other plant secondary compounds. Vet. Parasitol., 206: 322-327. [Crossref] [PubMed]

24. Tayo, G.M., Pone, J.W., Komtangi, M.C., Yondo, J., Ngangout, A.M. and Mbida, M. (2014) Anthelminthic activity of Moringa oleifera leaf extracts evaluated in vitro on four developmental stages of Haemonchus contortus from goats. Am. J. Plant Sci., 5: 1702-1710. [Crossref]

25. Sreelatha, S., Jeyachitra, A. and Padma, P.R. (2011) Antiproliferation and induction of apoptosis by Moringa oleifera leaf extract on human cancer cells. Food Chem. Toxicol., 49: 1270-1275. [Crossref] [PubMed]