Open Access
Research (Published online: 06-05-2018)
3. Prevalence of Cryptosporidium spp. oocysts in dogs in Lusaka district of Zambia
Lamson Mugala, Joyce Siwila, Ngonda Saasa and Girja Shanker Pandey
Veterinary World, 11(5): 585-589

Lamson Mugala: Department of Applied and Health Sciences, Biomedical Section, Evelyn Hone College of Applied Arts and Commerce, Lusaka, Zambia.
Joyce Siwila: Department of Clinical Studies, School of Veterinary Medicine, University of Zambia, P. O. Box, 32379, Lusaka, Zambia.
Ngonda Saasa: Department of Disease Control, School of Veterinary Medicine, University of Zambia, P. O. Box, 32379, Lusaka, Zambia.
Girja Shanker Pandey: Department of Disease Control, School of Veterinary Medicine, University of Zambia, P. O. Box, 32379, Lusaka, Zambia.

doi: 10.14202/vetworld.2018.585-589

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Article history: Received: 09-01-2018, Accepted: 03-04-2018, Published online: 06-05-2018

Corresponding author: Joyce Siwila


Citation: Mugala L, Siwila J, Saasa N, Pandey GS (2018) Prevalence of Cryptosporidium spp. oocysts in dogs in Lusaka district of Zambia, Veterinary World, 11(5): 585-589.

Aim: Cryptosporidium is one of the causes of diarrheal illness in man and animals worldwide and is zoonotic. The study aimed to determine the prevalence and risk factors associated with fecal shedding of Cryptosporidium oocysts in dogs in Lusaka district of Zambia.

Materials and Methods: A cross-sectional study was conducted in Lusaka district of Zambia during 2015-2016. A total of 390 dogs (243 males and 147 females) aged 2 months-13 years were enrolled. Fecal samples were collected and stained using modified Ziehl-Neelsen and Auramine O staining techniques and examined microscopically for oocysts.

Results: Overall, the prevalence of Cryptosporidium oocysts infection was 5.9% (23/390; 95% confidence interval: 3.9-8.7). Prevalence among male dogs and female dogs was 5.3% and 6.8%, respectively. Older dogs had a relatively higher infection rate compared to the younger puppies. There was a statistically significant difference in infection between nondescript breed and pure breeds with prevalence being higher in nondescript dog breeds. Water source was also significantly associated with Cryptosporidium infection.

Conclusion: Cryptosporidium infections are common, especially among the nondescript breed of domestic dogs in Lusaka district of Zambia. Further studies to characterize the common species are warranted.

Keywords: Cryptosporidium spp., dogs, Lusaka, prevalence, Zambia.


1. Samie, A., Tsipa, M.A. and Bessong, P. (2013) The epidemiology of Cryptosporidium in cats and dogs in the Thohoyandou region, South Africa. Afr. J. Microbiol. Res., 7: 2510-2518. [Crossref]

2. Aldeyarbi, H.M., Abu El-Ezz, N.M. and Karanis, P. (2016) Cryptosporidium and cryptosporidiosis: The African perspective. Environ. Sci. Pollut. Res. Int., 23: 13811-13821. [Crossref] [PubMed]

3. Squire, S.A. and Ryan, U. (2017) Cryptosporidium and Giardia in Africa: Current and future challenges. Parasit. Vectors, 10: 195. [Crossref] [PubMed] [PMC]

4. Tangtrongsup, S., Scorza A.V., Reif, J.S., Ballweber, L.R., Lappin, M.R. and Salman, M.D. (2017) Prevalence and multilocus genotyping analysis of Cryptosporidium and Giardia isolates from dogs in Chiang Mai, Thailand. Vet. Sci., 4: 26. [Crossref] [PubMed] [PMC]

5. Ramirez, N.E., Ward, L.A. and Sreevatsan, S.A. (2004) Review of the biology and epidemiology of cryptosporidiosis in humans and animals. Microb. Infect., 6: 773-785. [Crossref] [PubMed]

6. Santin, M. and Trout, J.M. (2008) Companion animals. In: Fayer, R. and Xiao, L., editors. Cryptosporidium and Cryptosporidiosis. CRC Press, Boca Raton, FL, USA. p437-450.

7. Uehlinger, F.D., Greenwood, S.J., McClure, J.T., Conboy, G., O'Handley, R. and Barkemae, H.W. (2013). Zoonotic potential of Giardia duodenalis and Cryptosporidium spp. and prevalence of intestinal parasites in young dogs from different populations on Prince Edward Island, Canada. Vet. Parasitol., 196: 509-514. [Crossref] [PubMed]

8. Bowman, D.D. and Lucio-Foster, A. (2010) Cryptosporidiosis and giardiasis in dogs and cats: Veterinary and public health importance. Exp. Parasitol., 124: 121-127. [Crossref]

9. Xiao, L., Cama, V.A., Cabrera, L., Ortega, Y., Pearson, J. and Robert, H. (2007) Possible transmission of Cryptosporidium canis among children and a dog in a household. J. Clin. Microbiol., 45: 2014-2016. [Crossref] [PubMed] [PMC]

10. Daniels, M.E., Shrivastava, A., Smith, W.A., Sahu, P., Odagiri, M., Misra, PR., Panigrahi, P., Suar, M., Clasen, T. and Jenkins, M.W. (2015) Cryptosporidium and Giardia in humans, domestic animals and village water sources in rural India. Am. J. Trop. Med. Hyg., 93: 596-600. [Crossref] [PubMed] [PMC]

11. Gbemisola, M.O., Beatty, V.M. and Gbeminiyi, R.O. (2016) Prevalence and risk factors associated with faecal shedding of Cryptosporidium oocysts in dogs in the federal capital territory, Abuja, Nigeria. Vet. Med. Int., 16. Article ID 4591238, 6 Pages.

12. Scorza, A.V., Duncan, C., Miles, L. and Lappin, M.R. (2011) Prevalence of selected zoonotic and vector-borne agents in dogs and cats in Costa Rica. Vet. Parasitol., 183: 178-183. [Crossref] [PubMed]

13. Geurden, T., Goma, F.Y., Siwila, J., Phiri, I.G.K., Mwanza, A.M., Gabriel, S., Claerebout, E. and Vercruysse, J. (2006) Prevalence and genotyping of Cryptosporidium in three cattle husbandry systems in Zambia. Vet. Parasitol., 138: 217-222. [Crossref] [PubMed]

14. Siwila, J. and Mwape, K.E. (2012) Prevalence of Cryptosporidium spp. and Giardia duodenalis in pigs in Lusaka, Zambia. Onderstepoort J. Vet. Res., 79: E1-5. [Crossref] [PubMed]

15. Siwila, J., Phiri, I.G.K., Enemark, H.L., Nchito, M. and Olsen, A. (2013) Occurrence of Cryptosporidium and Giardia in domestic animals in peri-urban communities of Kafue district, Zambia. Tanzania Vet. J., 28: 49-59.

16. Martin, S.W., Meek, A.H. and Willeberg, P. (1987) Veterinary Epidemiology, Principles and Methods. Iowa State University Press/AMES, IOWA 50010. p35-38.

17. Garcia, L.S. (2007) Diagnostic Medical Parasitology. 5th ed. ASM Press, Washington DC. p813-816.

18. Chadwick, C.S., Mcentegart, M.G. and Nairn, R.C. (1958) Fluorescent protein tracers: a trial of new fluorochromes and the development of an alternative to fluorescein. Lancet, 271: 412-414. [Crossref]

19. Siwila, J., Phiri, I.G.K., Enemark, H.L., Nchito, M. and Olsen, A. (2011) Seasonal prevalence and incidence of Cryptosporidium spp. and Giardia duodenalis and associated diarrhoea in children attending pre-schools in Kafue, Zambia. Trans. Roy. Soc. Trop. Med. Hyg., 105: 102-108. [Crossref] [PubMed]

20. Shukla, R., Giraldo, P., Kraliz, A., Finnigan, M. and Sanchez, A.L. (2006) Cryptosporidium spp. and other zoonotic enteric parasites in a sample of domestic dogs and cats in the Niagara region of Ontario. Can. Vet. J., 47: 1179-1184. [PubMed] [PMC]

21. Jian, F., Qi, M., He, X., Wang, R., Zhang, S., Dong, H. and Zhang, L. (2014) Occurrence and molecular characterization of Cryptopsoridium in dogs in Henan province, China. BMC Vet. Res., 10: 26. [Crossref] [PubMed] [PMC]

22. Zelalem, G. and Mekonnen, A. (2012) Prevalence of gastrointestinal helminths among dogs in Bahir Dar Town, Ethiopia. World Appl. Sci. J., 19: 595-601.

23. Ramirez-Barrios, R.A., Barboza-Mena, G., Mu-oz, J., Angulo-Cubillan, F., Hernandez, E., Gonzalez, F. and Escalona, F. (2004) Prevalence of intestinal parasites in dogs under veterinary care in Maracaibo, Venezuela. Vet. Parasitol., 121: 11-20. [Crossref] [PubMed]

24. Adejimi, J.O. and Osayomi, J.O. (2010) Prevalence of intestinal protozoan parasites of dogs in Ibadan, south western Nigeria. J. Anim. Plant Sci., 7: 783-788.

25. Awadallah, M.A. and Salem, L.M. (2015) Zoonotic enteric parasites transmitted from dogs in Egypt with special concern to Toxocara canis infection. Vet World. 8: 946-957. [Crossref] [PubMed] [PMC]

26. Nchito, M., Kelly, P., Sianongo S., Luo, N.P., Feldman, R., Farthing, M. and Baboo, K.S. (1998) Cryptosporidiosis in urban Zambian children: An analysis of risk factors. Am. J. Trop. Med. Hyg., 59: 435-437. [Crossref] [PubMed]

27. Kelly, P., Baboo, K.S., Ndubani, P., Nchito, M., Okeowo, N.P., Luo N.P., Feldman, R.A. and Farthing, M.J.G. (1997) Cryptosporidiosis in adults in Lusaka, Zambia, and its relationship to oocyst contamination of drinking water. J. Infect. Dis., 176: 1120-1123. [Crossref] [PubMed]

28. Korich, D.G., Mead, J.R., Madore, M.S., Sinclair, N.A. and Sterling, C.R. (1990) Effects of ozone, chlorine dioxide, chlorine, and monochloramine on Cryptosporidium parvum oocyst viability. Appl. Environ. Microbiol., 56: 1423-1428. [PubMed] [PMC]

29. Abere, T., Bogale, B. and Melaku, A. (2013) Gastrointestinal helminth parasites of pet and stray dogs as a potential risk for human health in Bahir Dar town, north-western Ethiopia. Vet. World, 6: 388-392. [Crossref]