In this study, we identified characteristics of systemic inflammation associated with surgical sepsis in animals. We evaluated the role of purine metabolism, functionally associated lipoperoxidation processes of membrane structures, and the antioxidant system in the development of surgical sepsis in dogs. Dogs with a provisional exclusion of sepsis were included in the study. The control group (Group 1) included clinically healthy dogs (n=5), and medium-breed dogs with systemic inflammation response syndrome (n=30) were categorized in the experimental group (Group 2). Along with hemogram and biochemical analysis, we determined the amount of malondialdehyde, glutathione, superoxide dismutase, catalase, glutathione reductase, and glucose-6-phosphate dehydrogenase on the 1st and 14th day of the study. Treatment included a thorough reorganization of the septic focus, followed by antibacterial therapy. Sick animals were injected with a drug (dexamethasone) that suppresses the synthesis and inhibits the action of inflammatory mediators. Decompensation of the functions of organs and systems was carried out using symptomatic therapy. We found that enhanced lipid peroxidation of unsaturated fatty acids of membrane structures stimulates the generalization of inflammatory process, as evidenced by the significant deviation from the physiologically normal values of lipid peroxidation, C-reactive protein, blood cell count, etc. The course of systemic inflammation associated with surgical sepsis in animals can be attributed to several consistently developing processes that function as a result of increased purine mononucleotide catabolism, peroxide compound formation, and their excessive breakdown in reactions associated with the consumption of glutathione due to the insufficient recovery of glutathione disulfide. The amount of uric acid, glycosaminoglycans, hyaluronic acid in blood plasma, and the content of malondialdehyde, glutathione, and glutathione reductase in erythrocytes should be considered when assessing the severity of the systemic inflammatory process. The increased glutathione requirement in dogs with surgical sepsis requires intervention with pharmacological agents, and further research is needed in this aspect. Keywords: dogs, purine mononucleotides, surgical sepsis.

The work aimed at studying the serological and clinical factors, as well as the risk factors of the Newcastle disease (ND) on broilers herds in Algeria. A sample of 1248 birds was randomly selected from 52 broiler flocks. We took blood samples from each bird at the level of the wing vein area where an indirect enzyme-linked immunosorbent assay technique was carried out through the use of an IDvet kit. The flocks showed 82.69% of seroprevalence. Clinically speaking, the most common symptoms were sneezing, rale, greenish diarrhea, torticollis, and motor discords. Most commonly observed postmortem lesions were the proventriculitis, tracheitis, and enteritis. Especially, the caeca are hemorrhagic. The scores show the effect of risk factors. There was a significant effect on the mortality, the hygiene and vaccination groups on antibody titers in time 2. The antibody titers were elevated in the herd that recorded a high mortality (more than 10%) compared with those which recorded a low mortality (<10%) (p=0.002). Therefore, the antibody titers were elevated in herds with bad hygiene, compared with the ones with good hygiene (p=0.04). At last, when broiler chicken were not boosted by ND vaccine, flocks appeared to be more seropositive (p=0.02). The serological survey conducted in this study provided an important scope for ND as a dominant viral disease in broilers. Many factors are responsible for the onset of these diseases; correct biosecurity measures are needed to reduce the impact of this pathology in poultry farms. Keywords: Algeria, biosecurity, enzyme-linked immunosorbent assay, Newcastle disease, serological, vaccination.

The current vaccination for peste des petits ruminants virus (PPRV) is stalled by myriad challenges and continuous endemicity of pneumonia due to fulminant bacterial complication in goats. The present study evaluated the protective effect of intranasal PPRV linage 1 and bacterine vaccinations. Twelve West African Dwarf (WAD) goats aged 6 months were randomly grouped and vaccinated within 2 weeks using a combination of PPRV lineage 1 vaccine (Nig/75), and bacterin from Mannheimia haemolytica (Mh) or Pasteurella multocida intranasally. The goats were observed for 3 weeks post-vaccination before comingled with a known infected WAD goat with apparent clinical signs of peste des petits ruminants and further observed clinically for 5 weeks post-infection (PI). Blood samples were taken for hematology while sera were assayed for antioxidants (glutathione peroxidase, glutathione transferase, and superoxide dismutase) activities and pro-oxidants (malondialdehyde content, reduced glutathione, hydrogen peroxide generation, and myeloperoxidase) using spectrophotometric methods. Data were subjected to parametric statistics at α=0.05 using GraphPad Prism version 21. Clinically, there were pyrexia, oculonasal discharge, diarrhea, anemia, leukopenia, and increased pro-oxidants in the unvaccinated goats, while moderate neutrophilia and leukocytosis were observed in PPRV and bacterin vaccinated goats. Two unvaccinated goats were weak and euthanized at 13 and 28 days PI. The goats vaccinated with PPRV and Mh showed better response clinically and biochemically. The mucosal vaccination of goats with PPRV vaccine and bacterine will protect against exposure and culminate in the development of protective mucosal, humoral, and cell-mediated immune responses. This vaccination strategy will provide framework needed in the prevention and control of endemic caprine pneumonia in Nigeria. Keywords: bacterines, goats, mucosal immunity, peste des petits ruminants virus.

The research was conducted to determine the seroprevalence and risk factor associated with respiratory viral pathogens in dual-purpose cattle of Aguachica, Rio de Oro and La Gloria municipalities in Cesar department, Colombia. The seroprevalence study was done from the random sampling (n=1000) of blood collected from 29 dual-purpose herds, located in three municipalities (Aguachica, Rio de Oro, and La Gloria) of Cesar department. The presence of antibodies against bovine herpesvirus type 1 (BHV-1), bovine respiratory syncytial virus (BRSV), bovine viral diarrhea virus (BVDV), and bovine parainfluenza-3 virus (BPI-3V) in the samples was detected by indirect enzyme-linked immunosorbent assay. Epidemiological data were obtained using a questionnaire administered to the owner or manager of each herd. The overall highest seroprevalence was observed for BHV-1 (94.7%), followed by BRSV (98.6%), BVDV (35.2%), and BPI-3V (47.1%). Regarding the seroprevalence by municipalities, there was a statistical association (p<0.05) for BVDV; however, for BRSV, BHV-1, and BPI-3V, no statistical association was found (p>0.05) between seropositive values and the municipalities, indicating that animal was seropositive in similar proportions in the three municipalities. Female sex and older animals (>24 months) were a significant risk factor for BHV-1 and BPI-3V infection. Regarding the clinical signs, there was a statistical association (p<0.05) between the seropositive values of BVDV and most of clinical signs observed, except for abortion. This research confirms the high seroprevalence of the respiratory viral pathogens in nonvaccinated cattle within the study areas. Therefore, appropriate sanitary management practices and routine vaccination programs should be adopted to reduce the seroprevalence of these infectious agents. Keywords: bovine herpesvirus type 1, bovine parainfluenza-3 virus, bovine respiratory syncytial virus, bovine viral diarrhea virus, seroprevalence, viral diseases.

This study aimed to sequence the Cytochrome c oxidase (COX-1) gene sequence from mitochondrial DNA of Sarcoptes scabiei isolated from Lamongan goats and Mojokerto rabbits, align it with DNA isolated from Zi'gong rabbit (GenBank accession No. EU256389.1), and produce a phylogenetic analysis of S. scabiei COX-1 gene. S. scabiei mites were obtained from goats and rabbits, and DNA was extracted using QIAamp DNA Mini Kit. The forward and reverse primer sequences were designed based on the DNA sequence of an S. scabiei COX-1 gene isolated from the Zi'gong rabbit (5'-TCTTAGGGGCTGGATTTAGTATG-3' and 5'-AGTTCCTCTACCAGTTCCAC-3', respectively). To confirm sequencing output, the sequence resulting from the reverse primer was inverted and aligned to the sequence from the forward primer using Clone Manager Professional Version 9 for Windows (Scientific & Educational Software; http://www.scied.com). This alignment was subsequently used to build a phylogenetic tree, using the Neighbor- Joining method, in the MEGA6 program (https://www.megasoftware.net/). Polymerase chain reaction (PCR) products from S. scabiei isolates from Lamongan goats and Mojokerto rabbits produced bands of around 290 bp with 2% agarose gel electrophoresis. Comparing the DNA sequences of the S. scabiei COX-1 gene with those isolated from Lamongan goats and Mojokerto rabbits showed 99% homology. PCR products of the S. scabiei COX-1 gene isolated from Lamongan goats and Mojokerto rabbits were around 290 bp long. The sequences had more than 99% homology. The sequences of the COX-1 gene of S. scabiei from Lamongan goats and Mojokerto rabbits were relatively close to the sequence of the gene in S. scabiei obtained from various hosts according to National Center for Biotechnology Information data. Keywords: cytochrome c oxidase-1, East Java, goat, Indonesia, rabbit, Sarcoptes scabiei.

Data mining in medical sciences provides countless opportunities for demonstrating hidden patterns of a data set. These patterns can help general physicians and health workers in preventing diseases. This study aimed to forecast delay times in post-exposure prophylaxis (PEP) to human animal bite injuries in central Iran using a decision tree analysis. The data of 2072 human animal bite cases were collected from Centers for Disease Control and Prevention unit of Qom Provincial Health Center, Iran from January 2017 to December 2018. The information related to animal bite incidents, including the biting animal characteristics and data on the bitten humans, was obtained by investigating the epidemiological survey forms of human animal bites. The decision tree model was applied to forecast the delay time of receiving PEP. A delay of more than 48 h in the initiation of PEP was estimated among 12.73% of animal bite victims. The most important variables to predict delay time of receiving PEP were the species of biting animal, time and cause of animal bite occurrences in 24 h a day, respectively. Hence, the model showed a delay in the initiation of PEP if the biting animal was a cattle or, a carnivore, and the time of being bitten was from 7 am to 1 pm, or if the animal was carnivore and the time of being bitten was between 1 and 7 pm, and the cause of animal bite was playing with the animal. Based on the findings of the study on different variables affecting the initiation of PEP, the concepts related to animal bite and rabies, including the timely injection of anti-rabies vaccine to prevent rabies, it is a must to educate and train, all the people, especially housewives and students. Keywords: decision tree analysis, human animal bite injuries, Iran, post-exposure prophylaxis.

Infectious coryza is caused by Avibacterium paragallinarum. In Indonesia, this infection results in a 10%-40% decrease in egg production by laying hens. This study was conducted to determine the effectiveness of tetravalent coryza vaccine contained A. paragallinarum bacterin serovars A, B, C2, and C3; strain A-221, B-Spross, C2-Modesto, and C-3-Akko in layers based on antibody titer and clinical signs using a post-challenge test. Forty four-week-old Lohmanns strain chickens were used in this study. Forty chickens were divided into four groups for serological and challenge test: Group 1 (unvaccinated and challenged by A. paragallinarum serovar A), Group 2 (unvaccinated and challenged by A. paragallinarum serovar B), Group 3 (vaccinated and challenged by A. paragallinarum serovar A), and Group 4 (vaccinated and challenged by A. paragallinarum serovar B). Vaccination was done using the tetravalent vaccine in oil-emulsion adjuvant contained A. paragallinarum bacterin serovars A, B, C2, and C3; strain A-221, B-Spross, C2-Modesto, and C-3-Akko. Vaccination was performed at day 1 and booster was done at day 14. Blood serum was collected on days 0, 14, and 28 for the hemagglutination-hemagglutination inhibition (HI) test. The challenge test was given at day 29 through intranasal administration using A. paragallinarum serovars A-L2447 and B-L1710 approximately 6×108 CFU/mL. Clinical signs were observed for 14 days post-infection. At the end of the study, chickens were euthanized, and pathological features of the infraorbital sinus, facial skin, and trachea were recorded. Data analysis of antibody titers and pathological changes was performed descriptively, while clinical symptom scores were analyzed non-parametrically with the Mann–Whitney U-test using SPSS version 21. At days 14 and 28 post-vaccination, the antibody titer in Group 3 was 5 HI and 20 HI, respectively. However, the antibody titers in Group 4 at 28 days post-vaccination were 0 HI. Clinical observations, the vaccinated groups that were challenged with A. paragallinarum serovars A and B showed clinical symptoms on days 4 and 6 post-infection, namely mild unilateral facial edema and severe bilateral facial edema, respectively. Clinical signs in Groups 3 and 4 were less severe than in Groups 1 and 2 (p<0.05). Pathological examination findings supported clinical observations and serological testing. Tetravalent coryza vaccine in chickens has efficacy to protect against the challenge test of A. paragallinarum serovars A and B isolated from Indonesia. Keywords: Avibacterium paragallinarum, challenge test, clinical signs, serological test, vaccine.

Mannheimiosis or pneumonic pasteurellosis commonly occurs in small ruminants. Mannheimiosis is caused by Mannheimia haemolytica (M. haemolytica) a Gram-negative coccobacillus producing acute febrile and infectious condition resulting in death of animal if not diagnosed and treated promptly. M. haemolytica serotype A2 is a commensal of the nasopharynx, gaining access to the lungs when host defenses are compromised by stress or infection in small ruminants. Till date, there is a vast literature and research that has been conducted on the pathogenesis of M. haemolytica invariably on respiratory system and its related immune system and mechanisms. From the clinical point of view, infection or diseases involving vital organs will systemically affect the production and performance of the infected animal. Therefore, there is a huge gap of knowledge and research to answer the question whether there is any association between M. haemolytica infection with reproductive physiology and performance in small ruminants and how it affects the productivity level. This review will explore the possibilities of involvement and new potential research to be carried out to determine the involvement of male and female reproductive system with M. haemolytica infection among small ruminants.

The present study was carried out to determine load of total bacteria, Escherichia coli and Salmonella spp. in dairy farm and its environmental components. In addition, the antibiogram profile of the isolated bacteria having public health impact was also determined along with identification of virulence and resistance genes by polymerase chain reaction (PCR) under a one-health approach. A total of 240 samples of six types (cow dung - 15, milk - 10, milkers' hand wash - 10, soil - 10 water - 5, and vegetables - 10) were collected from four dairy farms. For enumeration, the samples were cultured onto plate count agar, eosin methylene blue, and xylose-lysine deoxycholate agar and the isolation and identification of the E. coli and Salmonella spp. were performed based on morphology, cultural, staining, and biochemical properties followed by PCR. The pathogenic strains of E. coli stx1, stx2, and rfbO157 were also identified through PCR. The isolates were subjected to antimicrobial susceptibility test against 12 commonly used antibiotics by disk diffusion method. Detection of antibiotic resistance genes ereA, tetA, tetB, and SHV were performed by PCR. The mean total bacterial count, E. coli and Salmonella spp. count in the samples ranged from 4.54±0.05 to 8.65±0.06, 3.62±0.07 to 7.04±0.48, and 2.52±0.08 to 5.87±0.05 log colony-forming unit/g or ml, respectively. Out of 240 samples, 180 (75%) isolates of E. coli and 136 (56.67%) isolates of Salmonella spp. were recovered through cultural and molecular tests. Among the 180 E. coli isolates, 47 (26.11%) were found positive for the presence of all the three virulent genes, of which stx1 was the most prevalent (13.33%). Only three isolates were identified as enterohemorrhagic E. coli. Antibiotic sensitivity test revealed that both E. coli and Salmonella spp. were found highly resistant to azithromycin, tetracycline, erythromycin, oxytetracycline, and ertapenem and susceptible to gentamycin, ciprofloxacin, and imipenem. Among the four antibiotic resistance genes, the most observable was tetA (80.51-84.74%) in E. coli and Salmonella spp. and SHV genes were the lowest one (22.06-25%). Dairy farm and their environmental components carry antibiotic-resistant pathogenic E. coli and Salmonella spp. that are potential threat for human health which requires a one-health approach to combat the threat. Keywords: one-health, antibiotic resistance genes, dairy farm, Escherichia coli, carbapenem resistance, Salmonella spp., virulence.

Estrogen activity, a central component of reproductive growth, is regulated by the receptor proteins, estrogen receptor alpha (ERα), and ER beta (ERβ) in chickens as in many other species. ERα expresses predominantly in gonads. Although the expression of ERα in embryonic gonads has been studied in detail, the expression of ERα in post-hatching male gonads has not been studied adequately. Therefore, the current research was conducted to determine the post-hatching changes in the expression of ERα in the left gonads of male chickens with age. Shaver Brown male chickens were raised and cared for according to the management guide and sacrificed at the intervals of 1, 4, and 8 weeks of age. The total RNA was extracted from the left gonads using the Trizol method and reverse transcribed using a pair of gene-specific primers. Following polymerase chain reaction amplification, the expression of ERα was quantified relative to the expression of the reference gene GAPDH. The results showed that ERα expression significantly increases with age at p=0.0032. However, the increment of ERα expression from week 1 to week 4 was 2.04-fold and from week 4 to week 8 was 1.39-fold, with the later age reflecting a diminishing pattern in the increment. These results differentiate the post-hatching ERα expression of the left gonads of male chickens increase with age but with a diminishing gradient that may support their reproductive functions in later stages of life. Keywords: age, chicken, estrogen receptor alpha, gene expression, testicular.

The effect of some variables on hydatidosis in animals slaughtered at Cairo and Giza abattoirs was investigated and the influence on serum biochemical parameters, antioxidant enzymes, and histopathological lesions caused by these parasites as a consequence was estimated. The effect of some variables on hydatidosis in 397 sheep, 401 cattle, 435 buffaloes, and 341 camels slaughtered at Cairo and Giza abattoirs was investigated, and the influence on serum biochemical parameters, antioxidant activity and histopathological lesions caused by these parasites as a consequence was estimated. The results revealed that 39 sheep (9.8%), 74 cattle (18.4%), 95 buffaloes (21.8%), and 79 camels (23.25%) were infected. Concerning age variations, 165 young and 232 adult sheep, 215 young and 186 adult cattle, 194 young and 241 adult buffaloes, and 112 young and 229 adult camels were examined. The prevalence of hydatidosis was higher in adult sheep, cattle, and camel; 32 (13.8%), 49 (26.3%), and 56 (24.5%) than the younger ones 7 (4.2%), 25 (11.6%), and 23 (20.5%), respectively. Two hundred and eighty-eight sheep, 171 cattle were examined during winter. However, 109 sheep, 230 cattle were examined during summer. Hydatidosis infection in sheep and cattle was higher in winter 26 (9.01%) and 47 (27.5%) than in summer 13 (11.9%) and 27 (11.7%), respectively. Out of 133 sheep and 128 camels slaughtered in El-Basatin abattoirs, 36 (15.3) and 38 (29.7%) showed higher prevalence than that from El-Warak and El-Moneib abattoirs. Comparing with the non-infected groups, alkaline phosphatase activity decreased in hydatid-infected animals, while cholesterol and liver enzymes activities increased. Total lipid and triglyceride levels decreased in infected camels. Glutathione peroxidase, superoxide dismutase, and catalase decreased in hydatid-infected animals. The disturbance in the biochemical parameters, liver enzymes, and the antioxidant activities was consistent with the pathological findings that indicated the risk of hydatidosis infection. Finally, this study clarified the variabilities of hydatidosis in Cairo and Giza abattoirs as a starting point for future studies in different regions in Egypt. Keywords: abattoirs, antioxidant activity, hydatidosis, prevalence.

This study aimed to evaluate the effect of dietary monosodium glutamate (MSG) on growth performance and blood biochemical parameters of rainbow trout (Oncorhynchus mykiss W.). A total of 200 trouts were allotted in five experimental groups (n=40) that for 60 days received feed supplemented with MSG at the following levels: 0% (K) or 0.5% (E) or 1% (F) or 3% (G) or 5% (H). The average initial weight of trouts was 116.68±1.51 g (K), 116.58±1.33 g (E), 116.43±1.39 g (F), 117.40±1.47 g (G), and 115.95±1.88 g (H). At the end of the trial, control fish (K) had the lowest live weight (242.90±3.87 g) compared to Groups E (252.70±5.69 g), F (250.93±4.82 g), G (248.25±4.98 g), and H (247.95±4.74 g). Feed conversion ratio (FCR) of control group (K) was higher (1.11±0.02) versus FCR values established in Groups E (1.03±0.01), F (1.04±0.02), G (1.07±0.03), and H (1.06±0.02). Albumin, aspartate aminotransferase, gamma-glutamyl transpeptidase, alkaline phosphatase, phosphorus, magnesium, and triglyceride exhibited statistically significant differences versus controls. The results from the study showed that dietary supplementation of rainbow trouts with MSG increased live weight and reduced FCR. The optimum level of MSG recommended for addition to feed was 0.5%. Keywords: blood biochemical parameters, growth performance, monosodium glutamate, rainbow trout.

This study aimed to evaluate the protective effect of edible bird's nest (EBN) supplement on the uteri of rats exposed to lead acetate (LA) toxicity. Five treatment groups were established as follows: Group 1 (C), which was given distilled water; Group 2 (T0), which was administered with LA (10 mg/kg body weight [BW]); and Groups 3 (T1), 4 (T2), and 5 (T3), which were given LA (10 mg/kg BW) plus graded concentrations of 30, 60, and 120 mg/kg BW of EBN, respectively. Rats were euthanized at week 5 to collect blood for superoxide dismutase (SOD) assay, and uterus for histomorphological study and expression analyses of epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), and proliferating cell nuclear antigen (PCNA). Results revealed that LA causes destruction of uterine lining cells and necrosis of uterine glands of exposed rats without EBN supplement while the degree of damage decreased among EBN treated groups; T3 showed the highest ameliorating effect against LA toxicity, as well as an increased number of uterine glands. Increased levels of SOD were also achieved in EBN supplemented groups than the controls. Results of immunohistochemistry showed significantly higher expressions of EGF, VEGF, and PCNA levels (p<0.05) in T3 compared to other treatments. EBN maintained upregulation of antioxidant – reactive oxygen species balance. The findings showed that EBN could ameliorate the detrimental effects of LA toxicity on the uterus possibly by enhancing enzymatic antioxidant (SOD) activity as well as expressions of EGF, VEGF, and PCNA with cell proliferation roles. Keywords: edible bird's nest, growth factors, lead acetate, proliferating cell nuclear antigen, superoxide dismutase, uterus.

The present study was carried out to find out the causative agent of exanthematous skin lesions in sheep maintained by Southern Regional Research Centre, Mannavanur, Kodai hills, Tamil Nadu. Polymerase chain reaction (PCR) with Orf virus (ORFV) B2L gene-specific primers was carried out by employing the total genomic DNA isolated from the scabs as the template. The ORFV isolates from Kodai hills were characterized by the use of bioinformatics tools. The amino acid identity of ORFV isolate 1 from Kodai hills is having 98.14%, 96.29%, and 83.59% identity with reference strains of ORFV, Pseudocowpox virus, and bovine papular stomatitis virus, respectively. Phylogenetic analysis revealed that ORFV isolates from Kodai hills clustered with the other ORFV isolates from different geographical areas of India. The etiological agent of exanthematous skin lesion among sheep of Kodai hills is ORFV. Keywords: B2L gene, Kodai hills, Orf virus, phylogenetic analysis, sheep.

The study aimed to evaluate the occurrence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in sheep breeding in a particular husbandry system called "Elevage en case" in Dakar, Senegal. Blood samples were collected from 278 sheep. Serum was harvested and used for analysis. For the detection of T. gondii antibodies, 278 sera were analyzed using the modified agglutination test, while the enzyme linked-immunosorbent assay was used on 174 sheep sera to look for N. caninum antibodies. This study showed that 29.4±6.8% of sheep carried both T. gondii and N. caninum antibodies. The overall prevalence was 60.1±5.7% and 41.9±7.3% for toxoplasmosis and neosporosis, respectively. For toxoplasmosis, the prevalence was higher in Gueule Tapée (63.3%) than in Medina (58.9%), but the variation was not significant (p=0.45). Regarding the age of animals, the prevalence was significantly higher in animals over 2 years old compared to those under 2 years old (p=0.002). For neosporosis, the prevalence was significantly higher in Medina (48.67%) than Gueule Tapée (16.7%) (p=0.001), but non-significant variation was noted according to animal age. The study showed that sheep reared in households have carried antibodies of T. gondii and N. caninum. The prevalence was high and it means that consumption of meat from these animals is risky if the meat is eaten undercooked. Keywords: Elevage en case, Neospora caninum, prevalence, Senegal, sheep, Toxoplasma gondii.

Salmonellosis is one of the most common foodborne bacterial diseases in the world. The great majority of Salmonella infections in humans are foodborne with Salmonella enterica and Salmonella Typhimurium accounting for a major part of the problem. The objective of this study was to investigate the presence of invA gene in strains of Salmonellae isolated from eggs and diarrheal swabs from human cases. In addition, the relationship between invA gene nucleotide sequences from different sources (human stool and egg samples) have been studied through phylogenetic tree. One hundred and seventy eggs (eggshell and its contents) and 160 stool swabs samples were collected from four poultry farms and medical hospital in Giza Governorate. The study reported the presence of two Salmonella strains in eggshell surface with an overall isolation rate of 1.2 and 0% of the egg content. Salmonella Enteritidis and Salmonella Typhimurium were isolated from eggshell surface with an incidence of 50% for each strain. Six salmonella strains were isolated from human stool with an incidence of 3.75%; the isolated strains are S. Typhimurium, S. Enteritidis, Salmonella Virchow, Salmonella Haifa, and Salmonella Kentucky with an incidence of 33.3%, 16.6%, 16.6%, 16.6%, and 16.6%, respectively. Among eight Salmonella strains, invA gene was detected with percentage of 50%. The phylogenetic analysis of the sequences invA gene, from two isolates included in this study and five isolates retrieved from GenBank showed that sequence from human, layer hens, egg, and water in the same clusters. Close relation between drinking contaminated water and layer hens and contaminated water is one such source. Keywords: invA gene, phylogeny, Salmonella.

Dicrocoelium dendriticum or small liver fluke often causes unnoticed clinical manifestations in cattle. For a live animal, its diagnosis is mainly based on the detection of eggs by coproscopic examination. The objective of this study was to determine the presence or absence of Dicrocoelium spp. never previously reported in the study area but also to establish its prevalence, as well as an association between dicrocoeliasis and sex, age and season of the year, and histological characteristics. The study was carried out in slaughterhouses of three districts (Bouira, Tizi-Ouzou, and Bejaia) from January 2017 to December 2017. To this end, of 4053 cattle, representing more than 10% of the total number of animals slaughtered, stool and bile samples were collected and a liver inspection was carried out to investigate lesions of distomial cholangitis. They were processed for histological analysis. The specimens were morphologically identified according to the orientation of the testicles, the length and width of the body, and the level of the maximum width of the body. The total prevalence of dicrocoeliasis obtained of the 4053 cattle inspected is 0.52% with a prevalence of 0.66% in Tizi-Ouzou, 0.54% in Bouira, and 0.27% in Bejaia. About 0.52% of livers had distomial cholangitis (21 of the 4053 livers examined had adult D. dendriticum and 15% had non-distomial cholangitis. About 0.25% of cattle had D. dendriticum eggs in the stool versus 0.52% of cattle had parasite eggs in the bile. Statistical analysis revealed no significant association between dicrocoeliasis infection and the season of the year (p>0.05). However, a significant association was found between dicrocoeliasis infection and sex and age of the animal (p<0.05); females and older animals are more likely to have dicrocoeliasis. Histological analysis of the fluke revealed an anterior positioning of the testicles with a slightly oblique tandem orientation, an average body length of 3.69 mm and an average body width of 1 mm. The maximum body width level is either in the middle of the fluke body or in the rear position. The histological study confirms that the collected fluke is D. dendriticum. Thus, this work reveals for the 1st time in Algeria the presence of D. dendriticum in three districts (Bouira, Tizi-Ouzou, and Bejaia). The results indicate that many cattle farms in the North Central Province of Algeria are infested with D. dendriticum. Keywords: bile, cattle, dicrocoeliasis, Dicrocoelium dendriticum, liver, stools.

Police dogs in Iraq have been working mostly as explosive detectors since 2003. The health problems of these dogs are unique and have not been reported in literature. This investigation assessed the prevalence of health problems in Police dogs referred to Baghdad Veterinary Hospital during 2015-2017. A total of 1220 police dogs that were referred to Baghdad Veterinary Hospital in 2015-2017 were studied. The dogs were mostly German Shepherd dogs (GSDs) or Belgian Malinois (BM), with an average age of 4.6 years. The dogs' health problems and some of their risk factors were studied. Congestive heart failure (CHF), babesiosis, various malignancies, and intestinal parasites were the most commonly diagnosed health problems, followed by general aging, bronchopneumonia, otitis, nutritional deficiencies, and anemia. GSDs were more prone to CHF, while BM had more diagnoses of malignancies. Age was associated with both health conditions. The presence of health problems in these working dogs highlights the need for a stricter and more organized preventive schedule to keep the dogs healthy and efficient at old age. Keywords: health problems, Iraq, police dogs.

Manipulating lighting colors and regimens is considered an effective mean for improving broiler productivity. The influence of red, blue, and white light-emitting diode (LED) was investigated using three different regimens of lighting and darkness; continuous 23 h light (L):1 h dark (D), continuous 18 h L:6 h D, and intermittent 16 h L:8 h D hours on the performance, carcass weight (CW), feed and water intake (WI), serum glucose (GLUCO), triglycerides (TG), and cholesterol (TC), intestinal bacterial load, growth and metabolic hormones, and efficiency of Newcastle disease (ND) vaccine. A total of 252 1-day-old Ross broilers on deep litter were divided into nine groups. The 1st, 4th, and 7th groups were exposed to continuous 23L:1D, the 2nd, 5th, and 8th groups were exposed to continuous 18L:6D, and the 3rd, 6th, and 9th groups were exposed to intermittent 16L:8D (4L:2D, 4 times) lighting regimen using red, blue, and white LED lights, respectively. A total of 1350 samples (225 sera, 225 swabs, and 900 organ samples) were collected. Blue LED group revealed a highly significant increase (p<0.01) in live body weight, body weight gain, performance index, CW, spleen, heart, and liver weights, and anti-ND antibody titer, as well as a highly significant decline (p<0.01) of feed intake, WI, GLUCO, TG, TC, growth hormone, insulin, tri-iodothyronine (T3), tetra-iodothyronine (T4), total bacterial count (TBC), and total Enterobacteriaceae count compared to red and white LED lights in all tested lighting regimens. Continuous 23L:1D and 18L:6D regimens were significantly (p<0.01) superior to intermittent 16L:8D in their influence on the performance, CW, biochemistry, hormonal profile, and bacterial load. The blue LED light associated with continuous 18L:6D or 23L:1D h regimen is highly recommended in broiler houses for their enhancing the productive performance, growth, and immunity. Keywords: broilers, light-emitting diode colors, metabolism, Newcastle vaccine, productive performance.

This study aimed to isolate and identify lactic acid bacteria (LAB) in wild Sumatran orangutans to provide more information about LAB diversity derived from Sumatran orangutan feces. Fecal sampling from three female orangutans, around 35 years old, was carried out in the wild forest areas at the research station of Suaq Belimbing Gunung Leuser National Park located in the South Aceh district. Orangutan fecal samples were taken in the morning when the orangutans first defecated. The orangutans were above the tree, which is approximately 12-15 m from the ground where feces were found. Fermentation testing using the API 50 CHL Kit showed that OUL4 isolates were identified as Lactococcus lactis ssp. lactis with an identity value of 73.5%. Homology analysis demonstrated that the OUL4 isolates have 93% similarity to Weissella cibaria, and phylogenetic trees constructed using Mega 7.0 also showed that OUL4 isolates are related to W. cibaria. These results show that there is a difference in identification between biochemical testing with API kits and molecular analyses on LAB isolates from wild Sumatran orangutans. Based on 16S rRNA gene homology, the OUL4 LAB isolates from wild Sumatran orangutans have 93% homology to W. cibaria. Keywords: 16S rRNA, API 50 CHL, lactic acid bacteria, orangutan, Pongo abelii, Weissella.

Pigeon feces are increasingly being implicated in the spread of bacterial pathogens such as Escherichia coli, Campylobacter, Salmonella, Listeria, and Chlamydia. Fungi are rarely investigated except for Cryptococcus that has emerged as an important pathogen in old people and immunosuppressed patients. This study investigated fungi in pigeon feces collected from Mafikeng, the North West Province of South Africa. Freshly dropped feces were collected and enriched in phosphate-buffered saline overnight at 48°C and then subcultured on Sabouraud's dextrose agar and incubated at 48°C for 2 weeks observing any fungal growth from day 2. The growths were picked up, DNA extracted, and polymerase chain reaction was done using the internal transcribed spacer primers. Fungi isolated included: Aspergillus (Aspergillus tubingensis), Cryptococcus (Cryptococcus albidus and Cryptococcus randhawai), Fusarium spp., and Rhodotorula (Rhodotorula mucilaginosa and Rhodotorula kratochvilovae). Most of these isolates are known opportunistic pathogens and have been isolated in clinical conditions elsewhere. Other isolates such as Graphium dubautiae, Myrmecridium schulzeri, Naganishia albida, Paecilomyces lilacinus, and Zygopleurage zygospora were not found to be of any human health significance. We, therefore, concluded that the presence of these opportunistic pathogens is a significant human health risk, especially in the face of the HIV/AIDS pandemic that results in immunosuppression. Keywords: chain reaction, fungi, opportunistic pathogens, pigeon feces, polymerase.

The National Institute of Veterinary Epidemiology and Disease Informatics is an animal science research institute under the aegis of the Indian Council of Agricultural Research. The intellectual property management system (IPMS) of the institute oversees technology creation, protection, and transfer/commercialization. This study reviews the effectiveness of the IPMS using traditional strengths, weaknesses, opportunities, and threats (SWOT) evaluation. A comprehensive repository was developed to compile the SWOT pertaining to the IPMS based on relevant document reviews and the inputs of experts and stakeholders. The repository was shared among scientists of the institute for rating. The rating process revealed the top ten key SWOT associated with the structure and operation of the IPMS. The weighted SWOT matrix technique was used to identify the best strategies to improve and develop the IPMS further. This included strategies derived from the best combinations of key strengths and opportunities (S-O strategies), key weaknesses and opportunities (W-O strategies), key strengths and threats (S-T strategies), and key weaknesses and threats (W-T strategies). The top-ranked strengths included "possession of patented technology" and "state-of-the-art biosafety laboratory facilities," while "lack of in-house faculty with legal expertise in intellectual property rights (IPR)"" and "lack of technology incubation facilities" were the key weaknesses. The key opportunities included "external funding for research projects" and "market demand for onsite diagnostic tools." The major threats were "lack of market for veterinary diagnostics" and "broadbased patents on research tools and technologies." The strengths of the system, such as a state-of-the-art biosafety laboratory and technology-marketing collaboration with Agrinnovate India Ltd., could be employed effectively to gain from the opportunities tendered by the market demand for on-site disease diagnostic tools (S-O strategies). The limitation arising from a dearth of technical staff could be overcome by technological backstopping through international linkages in the area of disease monitoring and surveillance. Funding from externally supported projects could also be utilized for recruitment of personnel (W-O strategies). Limitations arising from the combination of inadequate in-house IPR expertise and the threat arising from broadbased patents on research tools warrant vigilance (W-T strategies). Keywords: Indian Council of Agricultural Research, intellectual property management system, intellectual property rights, National Institute of Veterinary Epidemiology and Disease Informatics, strengths, weaknesses, opportunities, and threats analysis.

The objectives of this study were to investigate the prevalence of Yersinia enterocolitica in retail chicken meat, ground and processed beef meat, determine their virulence-associated genes, antimicrobial susceptibility pattern, molecular detection of extended-spectrum β-lactamases, and their capability of biofilm formation in vitro. A total of 210 samples (120 retail chicken meat, 30 ground beef, 30 beef burger, and 30 sausage samples) were collected from different retail chicken outlets and markets located at Mansoura city between December 2016 and April 2017. Meat samples were examined bacteriologically for the existence of Y. enterocolitica; bacterial colonies that displayed positive biochemical properties were subjected to polymerase chain reaction targeting 16 rRNA gene. Y. enterocolitica isolates were tested for their susceptibility to six antimicrobial agents using disk diffusion method. Uniplex PCR was used for screening Y. enterocolitica isolates for the presence of two virulence chromosome-associated genes (ail and yst), and β-lactamases (blaTEM and blaSHV). The capability of Y. enterocolitica to form biofilms was detected by tube method. Thirty Y. enterocolitica isolates (14.29%) were recovered including 19 (15.83%) isolates from chicken meat, 3 (10%) from ground beef, 5 (16.67%) from beef burger, and 3 (10%) from sausage samples. Regarding ail gene, it was detected in 6.67% (2/30), while yst gene detected in 20% (6/30) Y. enterocolitica isolates. About 80%, 70%, 63.33%, and 50% of Y. enterocolitica isolates were sensitive to ciprofloxacin, gentamicin, cefotaxime, and streptomycin, respectively, while 83.33% of Y. enterocolitica isolates were resistant to both ampicillin and cephalothin. Interestingly, 21 (70%) isolates had the capability of biofilms formation in vitro. Among the multidrug-resistant (MDR) strains, a significant difference (p<0.05) was found between MDR and biofilm formation. However, biofilm formation was correlated with the resistance of the isolates to β-lactam antimicrobials and the presence of β-lactam-resistant genes. The presence of Y. enterocolitica in chicken meat, ground and processed beef meat represents a significant health risk for meat consumers, which reflects the contamination of slaughterhouses and processing operations, therefore, strict hygienic measures should be applied to minimize carcasses contamination. Keywords: antimicrobial susceptibility, biofilm formation, virulence genes, Yersinia enterocolitica.

The welfare of animals kept in livestock production systems has raised concerns around the world. Adult dairy cattle require adequate rest and spend approximately 12 h/day lying down. This cross-sectional study aimed to determine the stall factors and management practices affecting cows' lying time, stall cleanliness, and cows' cleanliness (udder and upper leg), in smallholder dairy cows in Meru County of Kenya. A total of 106 milking cows from 73 farms were assessed for daily lying time and cleanliness. Data loggers were used to record the lying time of cows for 3 days. Stall, udder, and upper leg cleanliness were assessed using a 5-score system: 1 (very clean) to 5 (very dirty). Management information was acquired using a questionnaire that was administered face-to-face to the farmers in their native Kimeru language. Univariable and multivariable linear and logistic regression models were fit to determine factors associated with cows' lying time and dichotomized stall and cows' own cleanliness, respectively. The mean daily lying time was 10.9±2.2 h, and the mean stall cleanliness score was 2.4±1.0. The mean average cleanliness scores of the udder and upper legs were 1.9±0.7 and 2.5±1.1, respectively. Overall, 35% of the stalls were categorized as dirty (>2.5), whereas 13% and 47% of the cows had udder and leg cleanliness scores >2.5, respectively. From the final multivariable models (p<0.05), daily lying time increased by 1.0 h for cows older than 5.25 years versus younger cows. Conversely, lying time decreased by 1.0 h with stall cleanliness scores >2.5 and by 1.6 h with poorly positioned neck rails. In an interaction term, addition of new bedding at least once a day without removing stall manure at least once a day decreased the daily lying time of the cows by 1.5 h, whereas failure to add new bedding at least once a day but removing stall manure at least once a day decreased the lying time of the cows by 1.2 h. Farm-level risk factors for stall dirtiness (>2.5) included delayed cleaning of the alley (odds ratio [OR]=6.6, p=0.032), lack of bedding (OR=4.9, p=0.008), and standing idle and/or backward in the stall (OR=10.5, p=0.002). Stalls categorized as dirty (OR=2.9, p=0.041) and lack of bedding (OR=2.7, p=0.065) were cow- and farm-level risk factors for dirtiness of the udder (>2.5), respectively, whereas the stall being dirty (OR=2.3, p=0.043) was the only risk factor (cow level) for dirtiness of the upper legs (>2.5). It was recommended that farmers should pay attention to the specific factors identified regarding the stall design (e.g., neck rail position) and bedding/manure management that impact the cleanliness of cows and their lying time. Keywords: dairy cows, Kenya, lying time.

Lumpy skin disease (LSD), is a highly infectious viral disease of cattle, caused by LSD virus (LSDV) which belongs to the genus Capripoxvirus of family Poxviridae. In the summer of 2017, skin lesions suggestive of LSD were observed in cattle at several governorates in Egypt. This study aimed to detect LSDV in cattle specimens using rapid serological and molecular diagnostic assays. A total of 46 skin biopsies and uncoagulated blood samples were collected from cattle with LSD suggestive clinical signs, as well as 290 coagulated whole blood samples from cattle without skin lesion in different governorates in Egypt during the summer of 2017. Skin biopsies were used for virus isolation from the chorioallantoic membrane of 11-day-old specific pathogen-free embryonated chicken eggs (SPF-ECEs). LSDV was identified using conventional polymerase chain reaction (PCR), real-time PCR (RT-PCR), and fluorescent antibody technique (FAT) with specific hyperimmune serum against LSDV. Cattle sera were examined using indirect FAT (IFAT) and indirect enzyme-linked immunosorbent assay (ELISA). Skin nodules and sitfast lesions were significant clinical signs observed in all LSD suspect cattle. SPF-ECEs, from which positive isolations were made and it showed characteristic inflammatory and focal white pock lesions. The isolated viruses were identified as LSDV by FAT, conventional gel-based PCR, and RT-PCR. Among the skin biopsies and corresponding blood samples, LSDV-positive samples percentage were 39.13 and 36.95 by RT-PCR, followed 34.78 and 28.26 by conventional PCR and then 32.6 and 26.8 by FAT, respectively. The total positive percentage of LSDV antibody detected in cattle serum samples were 17.93 and 14.48 by indirect ELISA and IFAT. LSDV was detected and identified in skin biopsies and corresponding blood samples of naturally infected cattle, more LSDV-positive samples were detected by RT-PCR, followed by conventional PCR and then FAT. The indirect ELISA detected more antibody-positive samples than the IFAT from cattle serum samples. The RT-PCR assay is simple, sensitive, rapid, and reliable for the detection of LSDV in blood and skin nodule biopsies of suspected cattle. Keywords: enzyme-linked immunosorbent assay, indirect fluorescent antibody technique, lumpy skin disease, polymerase chain reaction, Poxviridae, real-time polymerase chain reaction.

Testis and epididymis are male reproductive organs that play an important role in spermatogenesis. These two organs are rich in the content of hormones and other molecules needed in the process of spermatogenesis which affect the quality of the spermatozoa. The objective of this study was to examine the effect of the administration of epididymis and testicular extracts and their combination on testosterone, pituitary adenylate cyclase-activating polypeptide (PACAP), and protamine 1 (PRM1) concentrations in the serum of male chicken. Twenty male chickens (broiler strain Cp707), aged 3 weeks and weighing 800-1000 g, were randomly divided into four different groups including a control group (T0) = injected with 1 ml normal saline and treatment groups: T1 = injected with 1 ml epididymis extract, T2 = injected with 1 ml testicular extract, and T3 = injected with a combination of 1 ml epididymis + 1 ml testicular extract. The experiment was conducted for 13 days and at the end of the study (day 14), the chickens were sacrificed to obtain the serum. Furthermore, the concentrations of testosterone, PACAP, and PRM1 were then measured by using an enzyme-linked immunosorbent assay technique. The concentrations of PACAP and PRM1 did not show a significant difference between treatment groups (T1, T2, and T3) and control group (T0) (p>0.05). However, the concentration of testosterone showed a significantly higher difference in a group injected with a combination of 1 ml epididymis and 1 ml testicular extracts (T3) compared to the control group (T0) (p<0.05). The administration of epididymis and testicular extracts and their combination did not affect the increase of PACAP and PRM1 concentration. However, a combination of these extracts significantly affects the increase of testosterone concentration in the serum of male chicken. Keywords: chicken, epididymis and testicular extracts, pituitary adenylate cyclase-activating polypeptide, spermatogenesis, testosterone, protamine 1.

A previous study divided Indonesian bovine viral diarrhea virus (BVDV)-1 into subgenotypes BVDV-1a to BVDV-1d based on the partial NS5B gene using strain Bega as reference for BVDV-1a. In fact, it is clustered into BVDV-1c with strain Bega-like Australia. BVDV genotyping has been done on isolates from Jakarta, West and Central Java, but East Java isolates have not been genotyped. This study aimed to analyze genetic variability and amino acid residues in the nucleotide-binding pocket of the NS5B gene from infected cattle. Samples were obtained from the Sera Bank originating from active and passive surveillance of cattle that had been tested for BVDV antigen from 2013 to 2017. Detection of the p80 antibody and BVDV genotyping was carried out using ELISA and nested-multiplex-polymerase chain reaction (PCR), respectively. We defined 15 nested PCR products for partial sequencing of NS5B. Those field samples were selected from each location and year using proportional calculation as a representative sample. Homological and phylogenetic analyses of the partial NS5B gene were performed using BLAST and MEGA version 6. Based on the phylogenetic tree analysis using 360 nucleotides as the partial NS5B gene, Indonesian BVDV-1 isolates from Central and East Java were subdivided to BVDV-1a (n=9), BVDV-1b (n=1), and BVDV-1c (n=5). In the present study, the homology of BVDV subgenotype -1a, -1b, and -1c was compared to the BVDV GenBank data and found 90-93%, 93%, and 92-95% respectively with the average pairwise distance of 0.207. A point mutation was shown at R283K of all BVDV isolates based on the sequence of three amino acid residues R283, R285, and I287 in the nucleotide-binding pocket as a part of the encoded RNA-dependent RNA polymerase. This study revealed the genetic variability of BVDV infecting cattle in Central Java and East Java, Indonesia, the subtypes BVDV-1a, BVDV-1b, BVDV-1c, and a point mutation at the R283K residue. Keywords: bovine viral diarrhea virus, NS5B gene, phylogenetic analysis, point mutation, subgenotype.

The present study was conducted to evaluate the quality of rooster sperm at 5°C after treatment with a diluent containing coconut water, fructose, and chicken egg yolk and stored the semen sample at 5°C. Ten semen samples from 10 healthy roosters were subjected to four different treatments. For the treatments, 0.2 ml fresh semen with a sperm concentration of 5.2X109 cell/ml was mixed with T0 (no diluent), T1 (0.34 ml coconut water and 6 μl fructose), T2 (0.274 ml coconut water, 0.12 ml egg yolk, and 6 μl fructose), and T3 (0.34 ml egg yolk and 6 μl fructose) solutions. Each treated solution was stored at 5°C and evaluated both macroscopically and microscopically. Macroscopically, semen volume, pH, and sperm concentration were evaluated. The microscopic sperm characteristics examined included total motility (i.e., rapid, medium, or slow), progressive and non-progressive motility, viability, and spermatozoa abnormalities noted at different storage times. The results showed that spermatozoa motility was under 40%. The results indicated that sperm viability significantly affected (p<0.05). The highest mean value of sperm viability on day 7 of storage was found after treatment with the T2 solution (46.100±0.5677%). Similarly, spermatozoa abnormalities were significantly lower after treatment with the T2 solution (6.680±1.702%). The addition of a diluent containing coconut water, egg yolk, and fructose helped in the better preservation spermatozoa motility, as well as viability for up to 7 days when the semen samples were stored at 5°C. Keywords: chicken, coconut water, diluent, egg yolk, sperm quality.

This study aimed to describe the clinicopathological and necropsy findings in neonatal dairy calves affected with right torsion of the abomasum. The history and findings of clinical examination, hematology and serum biochemical analyses and necropsy were described in six neonatal Holstein calves with a confirmed diagnosis of right torsion of the abomasum. Furthermore, a review of the literature was carried out using internet search engines such as PubMed and Google Scholar concerning abomasal displacement in calves. Only published papers in scientific and refereed journals were reviewed. Six neonatal Holstein calves (four females and two males) aged between 7 and 21 days were diagnosed with right torsion of the abomasum during necropsy. The calves were presented with peracute signs of anorexia, dehydration, abdominal pain, and abdominal distension. Hematology and serum biochemical analyses revealed hemoconcentration and azotemia, hyponatremia, hypochloremia, and hypokalemia. Abomasal torsion in neonatal calves must be placed on the deferential diagnostic list of calves suffering from peracute signs of abdominal pain and distension. Although the underlying etiopathological factors are not fully known, correction of nutritional mismanagement is required to prevent the condition. Keywords: abdominal surgery, abomasal diseases, Holstein calves, roughage feeding.

Avian reovirus (ARV) is a constraint to poultry industry in Bangladesh as a cause of several diseases in chickens, especially in broiler. However, the actual status of the viral infection is not known because the large-scale study is not conducted in this country. Therefore, this study aimed to check the presence and distribution of ARV-specific antibody in respect to area, types of chickens (broiler breeder, broiler, and layer), vaccination status, and age of chickens in Gazipur and Mymensingh districts of Bangladesh. A total of 276 chickens' blood samples were collected from two well-organized broiler breeder stock, seven broiler farms, and five layer farms located at two districts, namely Gazipur and Mymensingh of Bangladesh. Blood samples were collected from wing vein of the apparently healthy chickens using 3 ml of syringe and serum was harvested by keeping the syringe at room temperature in slanting position. The sera were transferred to the laboratory by maintaining the cool chain and further processing was performed by indirect enzyme-linked immunosorbent assay using ARV antibody test kit. The results of serological test revealed that an average of 39.5% seropositive against ARV was recorded in chickens of Gazipur and Mymensingh districts. Among these, chickens of Gazipur district had the highest seropositivity of 50.5% than Mymensingh (30.7%). With respect to vaccination status, the seropositivity of vaccinated chickens in both areas was 100% and non-vaccinated chickens was 50.5% in Gazipur and 30.7% in Mymensingh district, respectively. However, regarding age groups, the seropositivity was higher in the age of 4-6 weeks (64.5%). The present serological findings showed a higher prevalence of ARV-specific antibodies in broiler birds. It indicates that the poultry industries of Bangladesh are contaminated with ARV which may naturally be transmitted to chickens either vertically or horizontally. Keywords: avian reovirus, enzyme-linked immunosorbent assay, seropositive.

The Barb horse occupies a prominent place in the history, culture, and equestrian traditions of the Maghreb (Algeria, Morocco, and Tunisia). Although many studies on the breed standard and morphometry have been published, there are no studies on its fertility and reproductive potential. Thus, this work aimed to study the fertility of Barb, Arabian Purebred, and Thoroughbred horses in Algeria. A total of 168 stallions and 1202 mares at the Chaouchaoua Stud farm in Tiaret, Algeria, were included in the study. The reproductive performance during 1592 cycles over 10 consecutive mating seasons (2003-2012) was evaluated. Multivariate analysis with logistic regression was used to analyze the reproductive parameters such as the number of cycles operated, number of pregnant mares, pregnancy rate per cycle, seasonal pregnancy rate, and embryonic mortality rate, and to determine the influence of breed, stallion's age, and year of the study on reproduction. Statistical analysis showed that stallion breed was a significant influencing factor for the number of pregnant barren mares (Odds ratio [OR]=1.72; p=0.03; 95% confidence interval [CI]=1.05, 2.84) seasonal pregnancy rate (OR=1.40; p<0.001; 95% CI=1.29, 1.53). Additionally, the reproductive performance of the Barb stallion was superior to that of the Thoroughbred stallion. With regard to the significant influence of stallion's age of >5 years on the number of pregnant foaling mares and seasonal pregnancy rate, significant interactions were observed between the stallion's breed and age, and the pregnancy rate per cycle: in the Barb breed, the pregnancy rate per cycle increased with the age of the stallion, while in the Thoroughbred, it decreased with age. Moreover, a significant effect of the year of the study on the pregnancy rate per cycle and number of pregnant foaling mares was observed. In contrast, the number of cycles and embryonic mortality rate were not influenced by the breed and age of the stallion, or the year of the study. The Algerian Barb horse attained a similar level of fertility compared with that of the Arabian Purebred and Thoroughbred stallions depending on its age and reproductive performance. Keywords: age, Barb, breeding performance, breed, stallion.

Despite the importance of the global emergence of Vibrio parahaemolyticus infections worldwide, there has been scanty information on its occurrence in Malaysian seawaters and fish. This study aimed to determine the occurrence of V. parahaemolyticus isolates using polymerase chain reaction targeted at toxin operon gene, thermostable direct hemolysin (tdh), and tdh-related hemolysin genes and to determine antibiotic resistance pattern, genes, and plasmid profile of V. parahaemolyticus from Malaysian seawaters and fish. Samples were collected from four recreational beaches in Malaysia (Port Klang; Bachok; Port Dickson; and Mersing). Thiosulfate-citrate-bile salts-sucrose (TCBS) agar and chromogenic Vibrio agar were used for isolation and identification. Colonies with yellow color on TCBS and green color on chromogenic vibrio (CV) agar were considered to be V. parahaemolyticus and they were subjected to biochemical tests. All V. parahaemolyticus isolates were further subjected to identification using seven specific gene markers. Seventy-three Vibrio isolates were recovered. Only one gene tdh> from seawater isolates of Vibrio has high virulence gene percentage (95.23%). Two genes alkaline serine protease (asp) and (tdh) had high percentage of virulence (83.87% and 80.64%, respectively) from fish. Comparatively, fish isolates have a higher virulence percentage compared to seawater isolates. Only gene streptomycin resistance B (strB) from seawater had 100% of the resistance genes. All isolates were multi-antibiotic resistant. Seventeen antibiotic resistance patterns were observed. The isolates had plasmids of varying sizes ranging from 2.7 kb to 42.4 kb. Dendrogram based on antibiotic resistance patterns of V. parahaemolyticus isolates discriminated the isolates into three clusters. This study demonstrated the occurrence of pathogenic, multi-antibiotic-resistant V. parahaemolyticus strains in Malaysian coastal waters and fish, and this could constitute potential public health risks. Keywords: antibiotic resistance genes, plasmid profile, Vibrio parahaemolyticus, virulence genes.

This study was designed to investigate the prevalence, associated risk factors, and zoonotic implications of gastrointestinal parasites of dogs in Maiduguri, Borno State, Northeastern Nigeria. A total of 200 rectal fecal samples were collected from dogs in Maiduguri for coprological examination using the saturated sodium chloride floatation technique. Detection of eggs or oocyst was done on the basis of keys of identification of parasites based on the morphology and size of eggs or oocyst. The prevalence of gastrointestinal parasites of dogs was 31.5% (63/200) in Maiduguri. The prevalence of the infection was higher in young, male dogs kept outdoor in terms of age, sex, and management; the difference was statistically significant (p<0.05). The parasites detected in Maiduguri include Ancylostoma spp., Toxocara spp., Dipylidium spp., Isospora spp., and Taenia spp., with Ancylostoma spp. (16%) having the highest prevalence rate. Gastrointestinal parasites have high prevalence in Maiduguri and constitute potential risk to human health because all genera of parasites detected in the study area are of public health importance. Keywords: coprological examination, gastrointestinal parasites, prevalence, zoonoses.

Dairy animals play an important role in the Indian economy. Knowledge of the occurrence pattern of long bone fractures in bovine may help in strategizing the possibilities of treatment and prognosis. This study aimed to find out the comparative occurrence pattern of fractures in cattle and buffaloes. A total of 278 fractures of bovine (171 in cattle and 107 in buffaloes), presented to the Department of Veterinary Surgery and Radiology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India, during a study of 1 year, were investigated for the occurrence pattern, in relation to species, age, body weight, gender, bone involved, type of fracture (closed or open), and the presentation of bovine as standing or in recumbent state. The overall hospital occurrence of fractures in bovine was 4.24% and most of the fractures resulted from slipping and falling on hard floor. Of 278 fractures, the majority (90.28%) involved long bones (n=251; 103 buffaloes and 148 cattle). Forelimb fractures were recorded more common in buffaloes (64.08%), whereas the cattle suffered more of hind limb fractures (60.23%). Cattle also included 11 cases of bilateral fractures of metacarpal (n=3), tibia (n=1), radius and ulna (n=1), and mandible (n=6). Fracture of olecranon constituted 6.83% (n=19) and majority (n=15) were in buffaloes. The cattle had higher percent of open fractures (54.38%) as compared to that in buffaloes (17.76%). Marginally higher percentage of cattle (33.33%) suffering from fracture were recumbent as compared to buffaloes (23.36%) and femur fractures were found to be a leading cause of recumbency (100% in cattle and 75.00% in buffaloes). Species-specific differences in the occurrence pattern of fractures exist among cattle and buffaloes. Cattle are found to be more susceptible (1.5 times) to fractures as compared to buffaloes. The buffaloes are vulnerable to forelimb fractures while the cattle to the hind limb. As compared to forelimb, long bone fractures of hind limb are more commonly associated with recumbency in bovine. Cattle are more prone to mandible fractures and the open fractures of long bones as compared to buffaloes. Keywords: buffalo, cattle, fracture, incidence, long bone.

The study aimed to identify fatty acid synthase (FASN), LOC514211, and fat mass and obesity-associated (FTO) gene polymorphisms and to investigate their associations with milk traits in an Indonesian-Holstein dairy cow population. A total of 100 Indonesian-Holstein cows consisting of 50 heads (0th generation; G0) and 50 heads of their daughters (1st generation; G1) were used. Polymerase chain reaction-restriction fragment length polymorphism was performed to genotype three single nucleotide polymorphisms: rs41919985 in the FASN gene, rs42688595 in the LOC514211 gene, and g.1371T>A in the FTO gene. FASN rs41919985 was associated with milk protein percentage (p<0.05), FTO g.1371T>A was associated with milk fat percentage (p<0.05), and LOC514211 rs42688595 was not associated with any trait (p>0.05). Heterozygote variants showed a higher protein percentage for FASN and the highest fat percentage for FTO. These associations were consistent in the G0 and G1 populations. Our results indicate that the milk protein and fat percentages can be improved by increasing the frequency of the AG genotype of FASN and the AT genotype of FTO, respectively. Keywords: fat mass and obesity-associated, fatty acid synthase, Indonesian-Holstein cattle, LOC514211, milk traits.

This study aimed to investigate the prevalence and implication of extended-spectrum beta-lactamase (ESBL) producing and Class 1 integrons (int1) gene carriers Escherichia coli isolates that demonstrated multidrug resistance (MDR) phenotypes and was isolated from turkeys that suffered from respiratory manifestations. A total of 120 freshly dead turkey poults that suffered from respiratory manifestations, with a history of treatment failure at Hefna, Belbis, Sharqia (Egypt) were sampled. From each bird lung and liver were aseptically collected and transported for laboratory investigations. Examination of samples collected from 120 freshly dead turkey poults revealed the isolation of coagulase-positive staphylococci, coagulase-negative staphylococci, Campylobacter spp., Salmonella spp., Proteus spp., Pseudomonas spp., Klebsiella spp., and E. coli with the prevalence rates of 12/120 (10%), 30/120 (35%), 17/120 (14.2%), 5/120 (4.1%), 17/120 (14.2%), 6/120 (5%), 7/120 (5.8%), and 18/120 (15%), respectively. E. coli isolates were subjected for serotyping and characterization, while the rest of isolates were preserved to be investigated later in further studies. Serogrouping of E. coli isolates revealed the identification of O119, O6, O8, and O169, while 1/18 of isolate was untypable. Studying phenotypic antibiotic susceptibility profiles of isolates revealed that 18/18 (100%) of isolates demonstrated resistance against cefuroxime, tetracycline, and trimethoprim, 16/18 (88.9%) of isolates demonstrated resistance to amoxicillin/ clavulanic acid, enrofloxacin, and norfloxacin, 14/18 (77.8%) of isolates demonstrated resistance to doxycycline and ciprofloxacin, and 9/18 (50%) of isolates showed resistance to gentamycin. Double disk synergy test showed that 6/18 (33.3%), 8/18 (44.4%), and 13/18 (72.2%) of isolates demonstrated the phenotypic pattern of ESBL producers with cefepime, cefotaxime, and ceftriaxone, respectively. Genotypic attributes for beta-lactamase TEM gene and int1 gene were studied by polymerase chain reaction and revealed that 17/18 (94.4%) of isolates were positive for both genes. Embryo lethality test indicated that the 18 studied E. coli isolates were considered primary pathogens. The results revealed that 18/18 (100%) of E. coli isolates demonstrated MDR against three or more antibiotic groups, 9/18 (50%) of isolates showed extensive resistance against the nine tested chemotherapeutic agents from seven antibiotic groups. It is recommended to monitor the circulation of MDR and ESBL-producing pathogens in poultry production in a one health approach, as a preventive measure to mitigate the risk imposed on public health. Keywords: Class 1 integrons, Escherichia coli, extended-spectrum beta-lactamase, multidrug resistance, turkeys.