Open Access
Research (Published online: 20-12-2021)
9. Apigenin induces oxidative stress in mouse Sertoli TM4 cells
Sathaporn Jittapalapong, Thapanee Poompoung and Samak Sutjarit
Veterinary World, 14(12): 3132-3137

Sathaporn Jittapalapong: Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand.
Thapanee Poompoung: Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand.
Samak Sutjarit: Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand.

doi: www.doi.org/10.14202/vetworld.2021.3132-3137

Share this article on [Facebook] [LinkedIn]

Article history: Received: 04-08-2021, Accepted: 02-11-2021, Published online: 20-12-2021

Corresponding author: Samak Sutjarit

E-mail: cvtsms@ku.ac.th

Citation: Jittapalapong S, Poompoung T, Sutjarit S (2021) Apigenin induces oxidative stress in mouse Sertoli TM4 cells, Veterinary World, 14(12): 3132-3137.
Abstract

Background and Aim: Apigenin (API) is an estrogenic compound found in many plants. Sertoli cells reside in the testis and are a key target of environmental toxicants. This study aimed to examine the cytotoxicity, especially oxidative stress of API in mouse Sertoli TM4 cells.

Materials and Methods: Mouse Sertoli TM4 cells were treated with 50 and 100 μM API for 48 h. Cell viability, lactate dehydrogenase (LDH) activities, glutathione reductase (GR) activities, production of reactive oxygen species (ROS), and malondialdehyde (MDA) levels were evaluated using various assays.

Results: Treatment with API at both 50 and 100 μM decreased viability and GR activity but increased LDH activity, ROS production, and MDA levels in mouse Sertoli TM4 cells.

Conclusion: Exposure to API induced oxidative stress in mouse Sertoli TM4 cells.

Keywords: apigenin, malondialdehyde, oxidative stress, reactive oxygen species, TM4 cells.