Open Access
Research (Published online: 19-07-2022)
12. Determination of multidrug-resistant populations and molecular characterization of complex Klebsiella spp. in wild animals by multilocus sequence typing
Alessandra Tammy Hayakawa Ito de Sousa, Marco Túlio dos Santos Costa, Stefhano Luis Cândido, Herica Makino, Thais Oliveira Morgado, Lucas Avelino Dandolini Pavelegini, Edson Moleta Colodel, Luciano Nakazato and Valéria Dutra
Veterinary World, 15(7): 1691-1698

Alessandra Tammy Hayakawa Ito de Sousa: Microbiology Laboratory of the Veterinary Hospital, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil.
Marco Túlio dos Santos Costa: Microbiology Laboratory of the Veterinary Hospital, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil.
Stefhano Luis Cândido: Microbiology Laboratory of the Veterinary Hospital, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil.
Herica Makino: Microbiology Laboratory of the Veterinary Hospital, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil.
Thais Oliveira Morgado: Center for Medicine and Research of Wild Animals, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil.
Lucas Avelino Dandolini Pavelegini: Pathology Laboratory of the Veterinary Hospital, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil.
Edson Moleta Colodel: Pathology Laboratory of the Veterinary Hospital, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil.
Luciano Nakazato: Microbiology Laboratory of the Veterinary Hospital, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil.
Valéria Dutra: Microbiology Laboratory of the Veterinary Hospital, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil.

doi: www.doi.org/10.14202/vetworld.2022.1691-1698

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Article history: Received: 01-02-2022, Accepted: 26-05-2022, Published online: 19-07-2022

Corresponding author: Valéria Dutra

E-mail: valeriadutra.dutra@gmail.com

Citation: de Sousa ATHI, Costa MTS, Cândido SL, Makino H, Morgado TO, Pavelegini LAD, Colodel EM, Nakazato L, Dutra V (2022) Determination of multidrug-resistant populations and molecular characterization of complex Klebsiella spp. in wild animals by multilocus sequence typing, Veterinary World, 15(7): 1691–1698.
Abstract

Background and Aim: One of the most significant public health concerns is multidrug-resistant (MDR) microorganisms. Klebsiella spp. have been at the forefront of causing different types of infections such as bacteremia, urinary tract infections, pneumonia, enteritis, and sepsis in humans as well as animals. This study aimed to determine the genomic similarity between Klebsiella spp. isolated from wild animal samples and those described in the Institut Pasteur genomic database to verify the spread of resistant clones regionally in the state of Mato Grosso, and to compare the epidemiological data in different regions of Brazil and the world.

Materials and Methods: Isolates from various sites of injury in wild animals were identified by sequencing the 16S rRNA gene. Antimicrobial susceptibility testing was performed using the disk diffusion method to verify the resistance profile, and then, multilocus sequence typing was performed to verify the population structure and compare the isolates from other regions of Brazil and the world.

Results: Twenty-three sequence types (STs) were observed; of these, 11 were new STs, as new alleles were detected. There was no predominant ST among the isolates. All isolates were MDR, with high rates of resistance to sulfonamides, ampicillin, amoxicillin, and nitrofurantoin and low resistance to meropenem, imipenem, and amikacin.

Conclusion: Improving our understanding of the population structure of Klebsiella spp. in wild animals may help determine the source of infection during outbreaks in humans or animals, as the One Health concept emphasizes the interlinks between humans, animals, and environmental health.

Keywords: antimicrobial, Klebsiella, multidrug resistance, multilocus sequence typing, susceptibility, wild animals.