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Research (Published online: 23-12-2014)

16. Semen quality parameters, their inter-relationship and post-washing sperm attributes of Rhode Island Red roosters - R. Richard Churchil, P. Ezhil Praveena and Deepak Sharma

Veterinary World, 7(12): 1117-1122



   doi: 10.14202/vetworld.2014.1117-1122



R. Richard Churchil: Genome Mapping Laboratory, Central Avian Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India; Instructional Livestock Farm Complex, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Orathanadu, Tamil Nadu, India;

P. Ezhil Praveena: Division of Aquatic Animal Health and Environment, Central Institute of Brackishwater Aquaculture, # 75, Santhome High Road, R. A. Puram, Chennai, Tamil Nadu, India;

Deepak Sharma: Genome Mapping Laboratory, Central Avian Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India; Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India;


Received: 20-10-2014, Revised: 19-11-2014 Accepted: 22-11-2014, Published online: 23-12-2014


Corresponding author: R. Richard Churchil, e-mail:

Aim: The present experiments were conducted (a) to evaluate the semen attributes of older Rhode Island Red (RIR) roosters and the inter-trait relationships, (b) to test sperm washing and storage duration suitable for gene transfer experiments.

Materials and Methods: The semen characteristics of older RIR roosters were studied, and Pearson correlation analysis was done to demonstrate the inter-trait relationships. Progressive motility and percent live sperms were tested at different post-washing intervals to identify suitable sperm processing conditions for gene transfer experiments.

Results: The volume, appearance score, initial motility, sperm count and percent live and abnormal spermatozoa were 0.38 ml, 3.58, 80.34%, 4.03 × 109 sperms/ml, 83.18% and 4.52% respectively. Positive correlation was observed among appearance score, motility, live sperm and sperm count. Semen volume is negatively correlated with all the other characters except live sperms, whereas, percent abnormal sperms negatively associated with all the other traits. Significant (p<0.05) decrease in terms of motility and live sperm was recorded at 60 min post-washing.

Conclusion: The semen attributes of RIR roosters compares well with the other breeds of chicken. The appearance score can be used to assess fertility where microscopic evaluation facilities are limited. The sperm washing protocol tested in the experiment is suitable for gene transfer experiments.

Keywords: correlation, live sperm, post-washing motility, Rhode Island Red roosters, semen attributes.

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