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Research
(Published
online: 27-09-2014)
19.
Influence of controlled breeding
techniques on fertility and plasma progesterone, protein and
cholesterol profile in true anestrus and repeat breeding buffaloes
- K. K. Savalia, A. J. Dhami, K. K. Hadiya, K. R. Patel and N.
P. Sarvaiya
Veterinary World, 7(9): 727-732
doi:
10.14202/vetworld.2014.727-732
K. K.
Savalia:
Department of Animal Reproduction, Gynaecology and Obstetrics,
College of Veterinary Science and Animal Husbandry, Anand
Agricultural University, Gujarat, India;
ketan89862@gmail.com
A. J.
Dhami:
Department of Animal Reproduction, Gynaecology and Obstetrics,
College of Veterinary Science and Animal Husbandry, Anand
Agricultural University, Gujarat, India;
ajdhami@aau.in
K. K.
Hadiya:
Department of Animal Reproduction, Gynaecology and Obstetrics,
College of Veterinary Science and Animal Husbandry, Anand
Agricultural University, Gujarat, India;
kamleshhadiya@yahoo.co.in
K. R.
Patel:
Department of Animal Reproduction, Gynaecology and Obstetrics,
College of Veterinary Science and Animal Husbandry, Anand
Agricultural University, Gujarat, India;
ketanfriend007@gmail.com
N. P.
Sarvaiya: Reproductive Biology Research Unit, College of
Veterinary Science and Animal Husbandry, Anand Agricultural
University, Gujarat, India;
npsarvaiya@aau.in
Received:
13-05-2014, Revised:
05-08-2014, Accepted:
11-08-2014, Published online:
27-09-2014
Corresponding author:
A. J. Dhami, e-mail: ajdhami@aau.in
Abstract
Aim:
The aim was to evaluate the estrus response, conception rate
and plasma profile of progesterone, protein and cholesterol
following use of different hormonal protocols in anestrus and
repeat breeding buffaloes.
Materials and Methods: This study was carried out on 20 true
anestrus, 20 repeat breeders, and 10 normal cyclic buffaloes. Ten
anestrus buffaloes each were treated with standard controlled
internal drug releasing (CIDR) i/vg device and Ovsynch (GPG)
protocols with fix timed artificial insemination (FTAI), and blood
samples were obtained on day 0, 7, 9/10 (AI) of treatment and day
21 post-AI. Ten repeat breeding buffaloes with mature mid-cycle
palpable corpus luteum (CL) were treated with i/m injection of 25
mg prostaglandin F2α (PGF2α) with FTAI twice at 72
and 96 h later, whereas other ten repeat breeding buffaloes in
standing estrus were inseminated with simultaneous i/m injection
of buserelin acetate-gonadotropinreleasing hormone (GnRH) 20 μg.
10 buffaloes exhibiting first estrus within 90 days postpartum and
inseminated without any treatment served as normal cyclic control.
Blood samples were obtained on day of PG injection, day of AI and
day 21 post-AI for estimation of plasma progesterone, protein, and
cholesterol.
Results: CIDR and Ovsynch protocols resulted in 100 and 80%
induction of estrus with conception rates of 40 and 30% at induced
estrus, respectively, in anestrus buffaloes. Mid-cycle PGF 2α
treatment resulted in 90% estrus induction and 40% conception rate
at induced estrus, while Buserelin acetate-GnRH 20 μg injection at
AI resulted in 30% conception rate in repeat breeders. In normal
cyclic control group also, the first service conception rate was
30%. The mean plasma progesterone concentrations on day 0, 7, 9/10
(AI) of treatment and on day 21 post-AI were found to be
significantly (p<0.05) different in both CIDR and Ovsynch
protocols, being higher on day 7 (day of PG injection) and on day
21 post-AI than on day 0 and 9/10 (FTAI), which were near basal
levels. The mean plasma progesterone level was significantly
(p<0.01) higher on the day of initiation of mid-cycle PGF2α
treatment (3.81±0.67 ng/ml) in a repeat breeding buffaloes
suggesting luteal phase. The mean plasma P4
levels on day 21 post-AI were significantly (p<0.01) higher than
on the day of estrus in both repeat breeders and in normal cyclic
controls. The plasma P4 value on day 21
post-AI was significantly (p<0.01) higher in conceived than
non-conceived buffaloes in all five groups. The mean plasma total
cholesterol and total protein concentrations in anestrus and
repeat breeding buffaloes under different treatments did not vary
significantly between sampling days. However the cholesterol
content was significantly (p<0.05) lower (79.96±2.17 vs.
92.27±6.04 mg/dl) and protein higher (8.14±0.73 vs. 7.69±0.59
g/dl) in conceived than non-conceived animals. In both anestrus
and repeat breeding buffaloes, the values of cholesterol and
protein were significantly lower than in normal cyclic control
group (138.04±11.98 mg/dl and 7.82±0.11 g/dl, respectively).
Conclusion: The results showed that CIDR was
better than Ovsynch protocol in inducing fertile estrus in
anestrus buffaloes, while mid-cycle PG treatment was superior over
AI + GnRH in repeat breeders, and all four treatment protocols
significantly influenced plasma P4 profile, but not the protein or
cholesterol.
Keywords: anestrus, biochemical profile,
buffalo, conception rates, plasma progesterone, repeat breeder,
synchronization protocols.
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