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              Open Access  
Copyright: The authors. This article is an open access 
article licensed under the terms of the Creative Commons Attribution License 
 
 
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distribution and reproduction in any medium, provided the work is properly 
cited. 
 
              
              
              Research 
              
              
(Published online: 
              19-09-2015) 
              
              10. Incidence 
              and clinical vital parameters in primary ketosis of Murrah 
              buffaloes - Ankit Kumar, Neelesh Sindhu, Parmod Kumar, 
              Tarun Kumar, Gaurav Charaya, Surbhi, V. K. Jain and Sridhar 
              
              Veterinary World, 8(9): 1083-1087   
              
   
                
                
doi: 
              10.14202/vetworld.2015.1083-1087   Ankit 
              Kumar: 
              Department of Veterinary Medicine, Lala Lajpat Rai University of 
              Veterinary & Animal Sciences, Hisar - 125 004, Haryana, India;
              
              
              ankitk813@gmail.com Neelesh 
              Sindhu: 
              Teaching Veterinary Clinical Complex, Lala Lajpat Rai University 
              of Veterinary & Animal Sciences, Hisar - 125 004, Haryana, India; 
              drneeleshsindhu@gmail.com Parmod 
              Kumar: 
              Department of Veterinary Medicine, Lala Lajpat Rai University of 
              Veterinary & Animal Sciences, Hisar - 125 004, Haryana, India;
              
              
              pdhuran09@gmail.com Tarun 
              Kumar: 
              Teaching Veterinary Clinical Complex, Lala Lajpat Rai University 
              of Veterinary & Animal Sciences, Hisar - 125 004, Haryana, India;
              
              
              tarunvet@gmail.com Gaurav 
              Charaya: 
              Department of Veterinary Medicine, Lala Lajpat Rai University of 
              Veterinary & Animal Sciences, Hisar - 125 004, Haryana, India;
              
              
              gcharaya9@gmail.com Surbhi:
              
              Department of Veterinary Physiology and Biochemistry, Lala Lajpat 
              Rai University of Veterinary & Animal Sciences, Hisar - 125 004, 
              Haryana, India;
              
              
              surbhi804@gmail.com V. K. 
              Jain: 
              Department of Veterinary Medicine, Lala Lajpat Rai University of 
              Veterinary & Animal Sciences, Hisar - 125 004, Haryana, India;
              
              
              vkjainluvas@gmail.com Sridhar: 
              Department of Veterinary Medicine, Lala Lajpat Rai University of 
              Veterinary & Animal Sciences, Hisar - 125 004, Haryana, India;
              
              drsrishar_saxena@gmail.com   
              Received: 23-05-2015, Revised: 12-08-2015, Accepted: 17-08-2015, 
              Published online: 19-09-2015   
              
              
              Corresponding author:Neelesh Sindhu, e-mail: drneeleshsindhu@gmail.com 
 
              Citation:Kumar A, Sindhu N, 
              Kumar P, Kumar T, Charaya G, Surbhi, Jain VK, Sridhar (2015) 
              Incidence and clinical vital parameters in primary ketosis of 
              Murrah buffaloes, Veterinary World 8(9): 1083-1087. 
 
              Abstract 
 Aim:
              The present study was undertaken to ascertain the incidence 
              and clinical vital parameters in cases of primary ketosis in 
              Murrah buffaloes brought to teaching veterinary clinical complex, 
              Lala Lajpat Rai University of Veterinary and Animal Sciences, 
              Hisar and from adjoining villages of the district Hisar, Haryana, 
              India. 
              Materials and Methods: The investigation was conducted on 24 
              clinical cases (out of total 145 screened) of primary ketosis. The 
              diagnosis was confirmed on the basis of clinical signs and 
              significantly positive two tests for ketone bodies in urine (Rothera’s 
              and Keto-Diastix strip test). Data collected were statistically 
              analyzed using independent Student’s t-test.  
              Results: Overall incidence of disease in these areas was found 
              to be 16.55% and all the animals were recently parturited (mean: 
              1.42±0.14 month), on an average in their third lactation (mean: 
              2.38±0.30) and exhibited clinical signs such as selective anorexia 
              (refusal to feed on concentrate diet), drastic reduction in milk 
              yield (mean: 64.4±5.35%), ketotic odor from urine, breath, and 
              milk and rapid loss of body condition. All the clinical vital 
              parameters in ketotic buffaloes (body temperature, heart rate, 
              respiration rate, and rumen movements) were within normal range.
               
              Conclusion: Primary ketosis in Murrah 
              buffaloes was the most common seen in the third lactation, within 
              the first 2 months after parturition with characteristics clinical 
              signs and no variability in vital parameters. The disease has 
              severe effect on the production status of affected animal. 
               
              Keywords: buffaloes, incidence, Keto-Diastix 
              strip test, primary ketosis, Rothera’s test. 
 
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