Expression profiling of heat shock protein genes in whole blood of Romosinuano cattle breed

Vet World Vol.16 March-2023 Article-22

Research Article

Veterinary World, 16(3): 601-606

https://doi.org/10.14202/vetworld.2023.601-606

Juan Camilo Taborda-Charris¹, Roy Rodríguez-Hernández², María Paula Herrera-Sánchez², Heinner Fabian Uribe-García¹, Rafael J. Otero-Arroyo^3,4, Juan Sebastian Naranjo-Gomez¹, Kelly Johanna Lozano-Villegas^1,2, and Iang Schroniltgen Rondón-Barragín^1,2

1. Immunobiology and Pathogenesis Research Group, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Altos the Santa Helena, A.A 546, Ibagué 730006299, Colombia.
2. Poultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad del Tolima, Santa Helena Highs, Postal Code 730006299, Ibagué-Tolima, Colombia.
3. Grupo de Investigación en Reproducción y Mejoramiento Genético Animal, Facultad de Ciencias Agropecuarias, Universidad de Sucre, Sincelejo 700001, Sucre, Colombia.
4. Laboratorio de Reproducción Animal, Corporación de Ciencias Biotecnológicas, Embriotecno, Montería 230029, Córdoba, Colombia.

Background and Aim: Heat shock proteins are highly conserved proteins that work as molecular chaperones expressed in response to thermal stress. This study aimed to determine the expression profile of genes related to the heat stress response in whole blood obtained from the Romosinuano creole breed.

Materials and Methods: Real-time polymerase chain reaction was performed to analyze the transcript of hsp90, hsp70, hsp60, and hsf1 in the whole blood of Romosinuano under different temperature-humidity indices (THIs).

Results: The expression levels of the hsp70 and hsf1 genes at the high-THI level were higher (p = 0.0011 and p = 0.0003, respectively) than those at the low-THI level. In addition, no differences in the expression levels of the hsp60 and hsP90 genes were detected between the two THIs.

Conclusion: The overexpression of hsf1 and hsp70 genes play an important role in protecting cells from damage induced by heat stress. Keywords: climate change, external environment, heat shock proteins, heat stress, mRNA, temperature-humidity index.

Keywords: climate change, external environment, heat shock proteins, heat stress, mRNA, temperature-humidity index.

How to cite this article: Taborda-Charris JC, Rodríguez-Hernández R, Herrera-Sánchez MP, Uribe-García HF, Otero-Arroyo RJ, Naranjo-Gomez JS, Lozano-Villegas KJ, and Rondón-Barragán IS (2023) Expression profiling of heat shock protein genes in whole blood of Romosinuano cattle breed, Veterinary World, 16(3): 601-606.

Received: 21-11-2022 Accepted: 02-02-2023 Published online: 24-03-2023

Corresponding author: Iang Schroniltgen Rondón-Barragín E-mail: isrondon@ut.edu.co

DOI: 10.14202/vetworld.2023.601-606

Copyright: Taborda-Charris, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.