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18 articles matching “amplification”

01
Research Article17 May 2025Vol. 18 · Issue 5

A comparison of diagnostic methods for canine Ehrlichiosis: Microscopy and RNases hybridization-assisted amplification technology compared with the quantitative polymerase chain reaction

Sakchai Ruenphet

This study aimed to determine the prevalence of major canine vector-borne pathogens in Bangkok, Thailand during the 2024 rainy season and evaluate the diagnostic performance of microscopy and a novel RNase hybridization-assisted amplification (RHAM) test kit …

Read article ↗6 citations
02
Research Article08 Jan 2024Vol. 17 · Issue 1

Detection of feline immunodeficiency virus by neutral red-based loop-mediated isothermal amplification assay

Jatuporn Rattanasrisomporn

This study aimed to develop a one-pot loop-mediated isothermal amplification (LAMP) process integrated with neutral red (NR-LAMP) assay for detection of FIV proviral DNA.

Read article ↗10 citations
03
Research Article19 Nov 2023Vol. 16 · Issue 11

Development of a recombinase-aided amplification method combined with lateral flow dipstick assay to detect Porcine circovirus type 2

Preeda Lertwatcharasarakul

In this study, we developed a recombinase-aided amplification combined with lateral flow dipstick (RAA-LFD) assay to compare with polymerase chain reaction (PCR) and quantitative PCR (qPCR) in detecting PCV2 in suspected field samples.

Read article ↗2 citations
04
Research Article17 Sep 2023Vol. 16 · Issue 9

Designing one-step reverse transcriptase loop-mediated isothermal amplification for serotype O foot-and-mouth disease virus detection during the 2022 outbreak in East Java, Indonesia

Eduardus Bimo Aksono

This study aims to devise a one-step reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) technique and phylogenetic analysis to detect the serotype O FMDV outbreak in East Java.

Read article ↗1 citations
05
Research Article07 Nov 2020Vol. 13 · Issue 11

Comparison of colorimetric loop-mediated isothermal amplification kit and reverse transcription-polymerase chain reaction in the diagnosis of peste des petits ruminants in sheep and goats in Southeast Nigeria

Ijeoma Chekwube Chukwudi

This study aimed to compare the application of a commercial colorimetric loop-mediated isothermal amplification kit and reverse transcriptase-polymerase chain reaction in the diagnosis of PPR in sheep and goats

Read article ↗5 citations
06
Research Article26 Nov 2017Vol. 10 · Issue 11

Application of loop-mediated isothermal amplification assay in the detection of herpesvirus of turkey (FC 126 strain) from chicken samples in Nigeria

A. J. Adedeji

This study was designed to optimize and apply the use of loop-mediated isothermal amplification (LAMP) as an alternative to conventional polymerase chain reaction (PCR) for the detection of herpesvirus of turkeys (HVT) (FC 126 strain) in vaccinated and non-va…

Read article ↗5 citations
08
Research Article24 Jul 2024Vol. 17 · Issue 7

Molecular detection of toxoplasmosis in wild rats using loop-mediated isothermal amplification assay

Lucia Tri Suwanti

This study aimed to estimate the prevalence of toxoplasmosis in wild rats through molecular detection as an indicator of environmental contamination in Surabaya.

Read article ↗1 citations
09
Research Article17 Jun 2023Vol. 16 · Issue 6

Development of loop-mediated isothermal amplification for rapid detection of sporotrichosis caused by Sporothrix schenckii

Surachai Pikulkaew

This study aimed to develop a visual diagnostic assay that is less time-consuming and can be used by veterinarians to screen for sporotrichosis.

Read article ↗2 citations
10
Research Article11 May 2022Vol. 15 · Issue 5

Single-stranded DNA aptamer-based rolling circle amplification as anti-chicken Salmonella bacteriostatic

Samer Sadeq Hameed

This study aimed to test the use of single-stranded DNA aptamers in the form of RCA-p as a bacteriostatic to Salmonella in vitro

Read article ↗6 citations
11
Research Article12 Mar 2022Vol. 15 · Issue 3

Development of loop-mediated isothermal amplification-lateral flow dipstick as a rapid screening test for detecting Listeria monocytogenes in frozen food products using a specific region on the ferrous iron transport protein B gene

Wirawan Nuchchanart

This technique is applicable to the development of detection technologies for other pathogens in food products

Read article ↗19 citations
12
Research Article17 Apr 2018Vol. 11 · Issue 4

Genetic characterization of 11 microsatellite loci in Egyptian pigeons (Columba livia domestica) and their cross-species amplification in other Columbidae populations

Miho Inoue-Murayama

The information from this study should be useful for genetic characterization and for developing conservation programs of this important species

Read article ↗12 citations
13
Research Article07 Mar 2024Vol. 17 · Issue 3

Comparative analysis of post-operative rehabilitation approaches for medial patellar luxation in small-breed dogs

Chalika Wangdee

This study aimed to assess the effectiveness of post-operative rehabilitation following medial patellar luxation (MPL) surgical correction by comparing ES and LASER therapy when combined with exercise. This was compared with a control group that consisted sol…

Read article ↗7 citations
14
Research Article17 May 2023Vol. 16 · Issue 5

Examination of macroscopic and microscopic lesions in IBDV-infected organs and molecular characterization of IBDV VP1 gene fragments obtained from commercial broiler farms in Indonesia

The study aimed to obtain pathological lesion data from chickens suspected of IBD virus (IBDV) infection by gross pathology, confirm IBDV infection through molecular diagnostics, and genotype the VP1 gene fragments of circulating IBDV in the field.

Read article ↗2 citations
15
Research Article31 Oct 2022Vol. 15 · Issue 10

Molecular diagnosis of bovine tuberculosis on postmortem carcasses during routine meat inspection in Benin: GeneXpert® testing to improve diagnostic scheme

Ange-Régis Zoclanclounon

This study aimed to determine the accuracy of the currently used bovine TB diagnostic method at slaughterhouses in Benin

Read article ↗7 citations